LOCUS       sequence-167814-         815 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  lentiviral expression vector for MS2-AID*Delta fusion protein with 
            hygromycin B selectable marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Hess GT, Fresard L, Han K, Lee CH, Li A, Cimprich KA, Montgomery SB,
            Bassik MC
  TITLE     Directed evolution using dCas9-targeted somatic hypermutation in 
            mammalian cells.
  JOURNAL   Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4038.
  PUBMED    27798611
REFERENCE   2  (bases 1 to 815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Oct 31. doi: 10.1038/nmeth.4038."
COMMENT     Sequence Label: pGH335_MS2-AID*Delta-Hygro
FEATURES             Location/Qualifiers
     source          1..815
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             501..554
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 agaggcgtga cagtgctaca tccttttcac tggactttgg ttatcttcgc aataagaacg
       61 gctgccacgt ggaattgctc ttcctccgct acatctcgga ctgggaccta gaccctggcc
      121 gctgctaccg cgtcacctgg ttcatctcct ggagcccctg ctacgactgt gcccgacatg
      181 tggccgactt tctgcgaggg aaccccaacc tcagtctgag gatcttcacc gcgcgcctct
      241 acttctgtga ggaccgcaag gctgagcccg aggggctgcg gcggctgcac cgcgccgggg
      301 tgcaaatagc catcatgacc ttcaaagatt atttttactg ctggaatact tttgtagaaa
      361 accacggaag aactttcaaa gcctgggaag ggctgcatga aaattcagtt cgtctctcca
      421 gacagcttcg gcgcatcctt ttgcccctgt atgaggttga tgacttacga gacgcatttc
      481 gtacttgtac aggcagtgga gagggcagag gaagtctgct aacatgcggt gacgtcgagg
      541 agaatcctgg cccaaccatg aaaaagcctg aactcaccgc tacctctgtc gagaagtttc
      601 tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct ctccgagggc gaagaatctc
      661 gggctttcag cttcgatgtg ggagggcgtg gatatgtcct gcgggtgaat agctgcgccg
      721 atggtttcta caaagatcgc tatgtttatc ggcactttgc atccgccgct ctccctattc
      781 ccgaagtgct tgacattggg gagttcagcg agagc
//
