LOCUS       sequence-167815-         869 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  lentiviral expression vector for MS2-AID-Delta-Dead fusion protein 
            with hygromycin B selectable marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Hess GT, Fresard L, Han K, Lee CH, Li A, Cimprich KA, Montgomery SB,
            Bassik MC
  TITLE     Directed evolution using dCas9-targeted somatic hypermutation in 
            mammalian cells.
  JOURNAL   Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4038.
  PUBMED    27798611
REFERENCE   2  (bases 1 to 869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Oct 31. doi: 10.1038/nmeth.4038."
COMMENT     Sequence Label: pGH183_MS2-AID-Delta-Dead-Hygro
FEATURES             Location/Qualifiers
     source          1..869
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      16..25
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             25..411
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2-N55K
                     /note="binds with high affinity to a specific stem-loop 
                     structure in the viral RNA (Lim et al., 1994)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIY"
     CDS             466..486
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gtgtcgtgac gtacggccac catggcttca aactttactc agttcgtgct cgtggacaat
       61 ggtgggacag gggatgtgac agtggctcct tctaatttcg ctaatggggt ggcagagtgg
      121 atcagctcca actcacggag ccaggcctac aaggtgacat gcagcgtcag gcagtctagt
      181 gcccagaaga gaaagtatac catcaaggtg gaggtcccca aagtggctac ccagacagtg
      241 ggcggagtcg aactgcctgt cgccgcttgg aggtcctacc tgaacatgga gctcactatc
      301 ccaattttcg ctaccaattc tgactgtgaa ctcatcgtga aggcaatgca ggggctcctc
      361 aaagacggta atcctatccc ttccgccatc gccgctaact caggtatcta cagcgctgga
      421 ggaggtggaa gcggaggagg aggaagcgga ggaggaggta gcggacctaa gaaaaagagg
      481 aaggtggcgg ccgctggatc catggacagc ctcttgatga accggaggaa gtttctttac
      541 caattcaaaa atgtccgctg ggctaagggt cggcgtgaga cctacctgtg ctacgtagtg
      601 aagaggcgtg acagtgctac atccttttca ctggactttg gttatcttcg caataagaac
      661 ggctgccgcg tgcaattgct cttcctccgc tacatctcgg actgggacct agaccctggc
      721 cgctgctacc gcgtcacctg gttcacctcc tggagcccct gctacgactg tgcccgacat
      781 gtggccgact ttctgcgagg gaaccccaac ctcagtctga ggatcttcac cgcgcgcctc
      841 tacttctgtg aggaccgcaa ggctgagcc
//
