LOCUS       sequence-167819-         950 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  serotonin receptor 2C RNA isoform 1 with C-terminal flag tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Zhang Z, Shen M, Gresch PJ, Ghamari-Langroudi M, Rabchevsky AG, 
            Emeson RB, Stamm S
  TITLE     Oligonucleotide-induced alternative splicing of serotonin 2C 
            receptor reduces food intake.
  JOURNAL   EMBO Mol Med. 2016 Aug 1;8(8):878-94. doi: 10.15252/emmm.201506030.
  PUBMED    27406820
REFERENCE   2  (bases 1 to 950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.15252/emmm"; 
            journalName: "EMBO Mol Med"; date: "2016-08-1- 1"; volume: "8"; 
            issue: "8"; pages: "878-94"
COMMENT     Sequence Label: 5HT2C-RNA1-FLAG
FEATURES             Location/Qualifiers
     source          1..950
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      10..19
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(29..52)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      817..826
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(894..945)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(923..943)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ccttgctcac catggtggcg accggtggct tatcgtcgtc atccttgtaa tcacgcgtca
       61 tggtggcggc ggatccgggt ttgcagagtt ctcttcctcg gccgtattcc tcttgcagca
      121 cttcaggaaa tccagactta gtccaggcgg ttcctcggtg tggccgtgca gtaacatcaa
      181 agcttgtcgg cgcagaacgt agatggtcag gcaatacgta atcaccataa tcgtcagcgg
      241 tatgaagaaa gctacgaagg acccaataag aacgaaattt gggtcgttga gcacgcacgt
      301 cgtgttgttc acgaacacct tttcttcgtc cctcagtcca atcacaggga taggaactga
      361 tacaccgatc cagcgatata gcgcagaggt gcatgatgga cgctgttgaa aataaaacat
      421 ctaaagaaat ccagacgggg cacaaatatc taggtagtgg ccagacataa tcataaagga
      481 ttgccaggag agacaggggc atgacaagta gtcccactag catatcagca atggctaggg
      541 acattaagaa gtaattggtg gcattgtgca gtttcttttc catgcttact gccatgatca
      601 caaggatgtt gccacctatt gtcatgatta ttatgatgac gattgaaagt gctggccagt
      661 tttgtacccc gtctgggaat ttgaagcgtc caccatcgga ggtattgaaa atgtcagtta
      721 ctatagctgc tactgggctc acagaaatat cacattgcca aaccaatagg ccaattaggt
      781 gcacaaggaa tgaatgcacc gcattcctca ggttcaccat ggtggcggaa ttcgaagctt
      841 gagctcgaga tctgagtccg gtagcgctag cggatctgac ggttcactaa accagctctg
      901 cttatataga cctcccaccg tacacgccta ccgcccattt gcgtcaatgg
//
