LOCUS       sequence-16782-e        1355 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1999
REFERENCE   2  (bases 1 to 1355)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: L4417
FEATURES             Location/Qualifiers
     source          1..1355
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             28..741
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGXVXILVELDGDVNGPXFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAXPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     681..701
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(751..767)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(751..767)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(824..843)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(825..843)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(932..949)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tcttgggcga ttgggtccgg tagaaaaatg agtaaaggag aagaactttt cactggancc
       61 gtcncaattc ttgttgaatt agatggtgat gttaatgggc ccnaattttc tgtcagtgga
      121 gagggtgaag gtgatgcaac atacggaaaa cttaccctta aatttatttg cactactgga
      181 aaactacctg ttccatggcc aacacttgtc actactttct cttatggtgt tcaatgcttt
      241 tcaagatacc cagatcatat gaaacggcat gactttttca agagtgccnt gcccgaaggt
      301 tatgtacagg aaagaactat atttttcaaa gatgacggga actacaagac acgtgctgaa
      361 gtcaagtttg aaggtgatac ccttgttaat agaatcgagt taaaaggtat tgattttaaa
      421 gaagatggaa acattcttgg acacaaattg gaatacaact ataactcaca caatgtatac
      481 atcatggcag acaaacaaaa gaatggaatc aaagttaact tcaaaattag acacaacatt
      541 gaagatggaa gcgttcaact agcagaccat tatcaacaaa atactccaat tggcgatggc
      601 cctgtccttt taccagacaa ccattacctg tccacacaat ctgccctttc gaaagatccc
      661 aacgaaaaga gagaccacat ggtccttctt gagtttgtaa cagctgctgg gattacacat
      721 ggcatggatg aactatacaa atgcccgggg gatccactag ttctagagcg gccgccaccg
      781 cggtggagct cgaattcatc gatgatatca gatctgccgg tctccctata gtgagtcgta
      841 ttaatttcga taagccaggt taacctgcat taatgaatcg gcccacgcgc ggggagaggc
      901 ggtttgcgta ttgggccgct cttccgcttc cctcgctcac tgactcgctg cgctcggtcg
      961 ttcggctgcg gcgagcgggt atcagctcnc tccaaaggcg gnnaatacgg ttatccacng
     1021 aaatcngggg anaaccgcag gaaaagaaca tgtgagcaaa aggccngcna aaaggccccg
     1081 nanccggnaa aaagggccgc ggtggtgggg gtttttccna naggcnnccc cccccctgan
     1141 cnacnttcna aaaaancgac ncctcnagtt cgaagggggn nnnacccncc gggnactnaa
     1201 agaanccnng ggnnttnccc ccnggaaann ccncgggncc cccccgnttc ccaaccngnc
     1261 ncntacngga aacccggccc ccttttnccn nggggnaagg gggggcnttc ccnacccccc
     1321 cnnaggattc ccctcngggg aggnnnnccc ccccc
//
