LOCUS       sequence-167831-       10831 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses efflux pump in E. coli to provide resistance to biofuel 
            (low copy).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10831)
  AUTHORS   Turner WJ, Dunlop MJ
  TITLE     Trade-Offs in Improving Biofuel Tolerance Using Combinations of 
            Efflux Pumps.
  JOURNAL   ACS Synth Biol. 2015 Oct 16;4(10):1056-63. doi: 10.1021/sb500307w. 
            Epub 2014 Dec 12.
  PUBMED    25496359
REFERENCE   2  (bases 1 to 10831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500307w";
            journalName: "ACS Synth Biol"; date: "2015-10-16- 16"; volume: "4"; 
            issue: "10"; pages: "1056-63"
COMMENT     Sequence Label: pBbS5c-AcrB/AcrD/AcrFa, ABO_0964
FEATURES             Location/Qualifiers
     source          1..10831
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(49..1131)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     1093..1112
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(1132..1209)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     1415..1434
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        join(1520..1525,1526..1543,1544..1550)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    1558..1574
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      7574..7645
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      7661..7688
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(8219..9169)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     terminator      complement(9931..10025)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(10046..10705)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     10620..10639
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(10706..10808)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
ORIGIN
        1 gacgtcggtg cctaatgagt gagctaactt acattaattg cgttgcgctc actgcccgct
       61 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
      121 ggcggtttgc gtattgggcg ccagggtggt ttttcttttc accagtgaga cgggcaacag
      181 ctgattgccc ttcaccgcct ggccctgaga gagttgcagc aagcggtcca cgctggtttg
      241 ccccagcagg cgaaaatcct gtttgatggt ggttaacggc gggatataac atgagctgtc
      301 ttcggtatcg tcgtatccca ctaccgagat gtccgcacca acgcgcagcc cggactcggt
      361 aatggcgcgc attgcgccca gcgccatctg atcgttggca accagcatcg cagtgggaac
      421 gatgccctca ttcagcattt gcatggtttg ttgaaaaccg gacatggcac tccagtcgcc
      481 ttcccgttcc gctatcggct gaatttgatt gcgagtgaga tatttatgcc agccagccag
      541 acgcagacgc gccgagacag aacttaatgg gcccgctaac agcgcgattt gctggtgacc
      601 caatgcgacc agatgctcca cgcccagtcg cgtaccgtct tcatgggaga aaataatact
      661 gttgatgggt gtctggtcag agacatcaag aaataacgcc ggaacattag tgcaggcagc
      721 ttccacagca atggcatcct ggtcatccag cggatagtta atgatcagcc cactgacgcg
      781 ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg acgccgcttc gttctaccat
      841 cgacaccacc acgctggcac ccagttgatc ggcgcgagat ttaatcgccg cgacaatttg
      901 cgacggcgcg tgcagggcca gactggaggt ggcaacgcca atcagcaacg actgtttgcc
      961 cgccagttgt tgtgccacgc ggttgggaat gtaattcagc tccgccatcg ccgcttccac
     1021 tttttcccgc gttttcgcag aaacgtggct ggcctggttc accacgcggg aaacggtctg
     1081 ataagagaca ccggcatact ctgcgacatc gtataacgtt actggtttca cattcaccac
     1141 cctgaattga ctctcttccg ggcgctatca tgccataccg cgaaaggttt tgcgccattc
     1201 gatggtgtcc gggatctcga cgctctccct tatgcgactc ctgcattagg aagcagccca
     1261 gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg
     1321 cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca
     1381 tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag
     1441 caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga
     1501 tcgtttaggc accccaggct ttacacttta tgcttccggc tcgtataatg tgtggaattg
     1561 tgagcggata acaatttcag aattcaaaag atcttttaag aaggagatat acatatgtat
     1621 atactgaacc tgattcattt ttcatgcaag caccacaagc cgtctttaat ggacaagctg
     1681 atggcgtgct atagcgtgtt tccaagcact gaggtgatta tgcgcagcgg tttcattctc
     1741 cccctgatag caggcgtact cgcactgcag ctaagtggct gcggcgatgc cggccaatcc
     1801 ggccaacaac aacagcaaca agcacccgaa gtcggctttg tcaccgtcaa ggcggccacc
     1861 gtcaccttga accgggaact gcccgggcga accactgcac accaggttgc cgaagtgcgg
     1921 ccccaagtca gtggtgtgat agaaaagcgc ctctttgaag aaggccaaga agtaagcgcg
     1981 gggcaaccgc tgtacaaaat agactcacgc acctatcaag cagcggtggc tagcgcccga
     2041 gcggagctgg cccgtgccac tgccaccctg aattccaacg acttgcgagc caaacgcttc
     2101 aagaaactgt tggattacaa agcggtgagc cagcaagaat acgacgaagc tcaggcccag
     2161 ctggatgaga acaaggccgc cgtagccgct gcacgtgcca atctgcaaag tgcccagatc
     2221 aatctggatt acgccacgat taaagcgcca atcagcgggc gcattggccg ctccagcgtc
     2281 accgccggtg ctttggtcac cgccaatcag gcaaacgccc tggccaccat tcgccaactc
     2341 gacccgatct acgtagacct gacccagtca agcaacgagc tgcgtcagct gcgccaagcc
     2401 atgcaggccg gtgagctgca acaggtcagc gacgacgaag cacgcattac cctaatactc
     2461 gaagatggct cccactataa ggaagccggt gccctgcaat tttccgaatt cgccgtggat
     2521 gaaagcaccg gctcagtcac cttgcgtgcc ctatttccca acccggacgg cgaactgctg
     2581 cccggcatgt tcgttcgcgg ccgcctaccg gaaggtcaac gcaaggatgc cattctggtt
     2641 ccccaaaaag gcattacccg cgacccgacc ggccaagcct acgccatgct gatcgacgac
     2701 aataatcagg taaaaaaagt gaaagtccgc actgagcgcg cagtcggcaa ccagtggctt
     2761 attgccgacg gactgaaaga cggtgatcgc ctgattgtgg acggcctgca acggattcag
     2821 cccggcgctc cggtttcccc agtggacaca gaaaaagcca acgtcgagcc cgcacccgca
     2881 cagcaagaac aaccccagtc ccccgccagc gacgctggcg aggcacaaag cgcagcgcaa
     2941 taatacgcaa cgacgagaaa cgggattccc atggccagat tttttattga ccgtccaatt
     3001 tttgcctggg tgattgccat catcatgatg atggccggtg ccttggccat ttacaccctg
     3061 cccattgagc agtaccccac cgttgcgcct ccgcaagtct ccattgccgg taactacccc
     3121 ggcgcttcgg cgaaaaccgt ggaagattcg gtcacccagg tgatcgagca acaaatgaac
     3181 gggatcgaca acctgctgta catgagctcc agcagtgact ctttcggtaa tgccgcagtg
     3241 aacctgacgt tcgcccccgg caccgacccg gacattgcgc aagtacaagt acagaacaag
     3301 ctacagctgg cgacccccct gctgccccag gaagtgcagc aacaaggaat gcaggtcacc
     3361 aaatcctcag actctttcct gatggtggcg ggctttacct ccgaagatgg cagcctatcc
     3421 cgtgctgacc tagccgatta cgtggcctcc aatgtgcagg acccggtcag ccgggtgccc
     3481 ggcgtgggtc agatccagct gttcggctct ccctatgcca tgcgcgtctg gctggacccg
     3541 aacaaactga ataaatttga tttaaccccc caagatgtta cccagacaat cgaggttcaa
     3601 aataatcagg tagccagcgg tcaattgggc ggcgcaccgg ccgtagaagg ccagcaattg
     3661 aacgccacca tcatcgccca gacccgcctg gaagacgtgg atcaattcga gaatatcctg
     3721 ctgaaagtaa acccggacgg ctctcgagtg ttccttaaag acgtggcccg agtcgaactg
     3781 gcagcgcaga actacgacgt gcagggccgt tataacggcc agcccgccgc cggcctggcc
     3841 atcagcctag ccaccggcgc caacgccctg gacaccgcag aagcactgcg tgcccgccta
     3901 gccgaactgc agccctactt ccctgataag atggaaatgg tgtttcccta cgacaccacg
     3961 cccttcgtca gtatctccat cgaagaagtg gtacataccc tgttcgaagc catcatcttg
     4021 gtcttcctgg tcatgtatct gttcttacaa aacttccgcg ccaccctcat ccccacacta
     4081 gcggtgcccg tggttctgct aggcaccttt gcggtgctgg ccgccttcgg cttttccatc
     4141 aataccctaa ccatgtttgg catggtgctg gccatcggcc tactggtaga tgacgccatc
     4201 gtggtggtgg aaaacgtgga acgggtgatg cacgaggagg gcctcccgcc caaagaggct
     4261 acccgtaaat ccatggggca gattacgggc gcgctggtgg gcatcgcctt ggtactgtca
     4321 gccgtattcg ttcccatggc ctttttcccg ggctccaccg gagccattta ccgccagttc
     4381 tccatcacca ttgtgtccgc catggtgctg tccgtgctgg tggccttgat cctcactccg
     4441 gccctgtgtg ccaccatgct caaggccaag gacagcgagc accaaaccga tcatggcttc
     4501 ttcggctggt ttaaccgcac cttcgacaag agcagccgtc gttatcaggg cagcgtggaa
     4561 aaaatcctcg gccgccgggg ccgctacctg tttatctatg tggtgatcgt cggtgtgctc
     4621 ggcttcagct tcatgcgcct tccatccagc ttcctgccgg aagaagatca ggggattttg
     4681 ttcaccctag tgaccctgcc agcgggctca acccaagagc agacagtcaa agtgctggaa
     4741 aaaatggagg actattatct aaatgaagag gcctccgctg tggatggcct gttcacggtg
     4801 gccggcttca gtttcaccgg ccgcggccag aacgccggca tggcgttcgt gaacctgaag
     4861 gactggtcag aacgggatct gtccgtggac ggcgccgaca atgtggttgc ccgcgccatg
     4921 ggctatttct ccaccattcg cgaagcgatg atgtttgcac tgaatccgcc atcgattcct
     4981 gaactgggca acgccagcgg gttcgacttc cagctgcttg accagtctgg tcaagggcac
     5041 gaagcgctga ttcaggctcg taaccagatg ctcggcatgg ccgcccaaga tcctcgactg
     5101 gccggggtac gccccaatgg tttggaagac agcccgcagt accaaatcga tattgaccag
     5161 cagaaagcca aagcgctagg gctgtccatc agcgacatta acagcactct gcaaatcgcc
     5221 tggggctcca gctacgtcaa taactttgtc gaccggggcc gggtaaaacg agtgtatgtg
     5281 caggccgatg ccccctaccg catgctaccg gaaaacgtca acgactggtt tgtgcgtaat
     5341 aaccaaggga aaatggtgcc gttctccacc tttgccacgg gccactggac gtatggttct
     5401 cccaagctag aacgctataa cggtgtgtcg tcggtgaaca tccaaggcaa cgcggcaccc
     5461 ggctacagta caggcgacgc catggatgcc atggaagaac tatccgctaa gctgcccgcc
     5521 gggttcggct tcgaatggac cggaatgtcc taccaggaac gtcagtctgg tgaccaggcc
     5581 cctgcgctgt atgtcatctc actattagta gtattcctct gtctggcggc cctgtatgaa
     5641 agctggtcga ttccattcgc ggtaatgctg gtggtccctc tgggcattct cggtgcggta
     5701 ctggccgcta ccttccgtga cctgaacaac gacgtcttct tccaggtggg gctgttaacc
     5761 accatcggcc tctcggcgaa aaacgccatt ctgattgcgg aattcgcgct ggagctggag
     5821 caaaaaggcg agcacttgct caaggccacc ctagaagccg tgcgcatgcg gctgcgcccg
     5881 atcctgatga cctcactggc cttcatgctc ggggtaaccc cactgatgat cagtactggt
     5941 gctggtgccg gtgctcgtaa cgccatcggt accggggtat tcggcggaat gctcaccgcc
     6001 accgtcctcg ccatcttctt cattccgctg ttctacgtgg cggtgcgcaa actatccggc
     6061 gtgcccctcg acggcaagaa gaaaggcaaa gaataagtcc acgcggagta atactgatga
     6121 cccgtaaccc actgacgctc gcccttctgg gcgggctgat gctgggtggc tgcaccctcg
     6181 cccccagcta tcaacaacca ctctctcccg tccctgcgca actgggcgaa gccaacggca
     6241 atagtgccag cgagctaacc ctgcccagct ggtccagcct ttatcagagc gcggaattgc
     6301 aacagctgat tcaaagcgca ttggataaca accgagatct gcgcatcgcc gtactcgatg
     6361 tggccgccct gcgcgcccag taccaaatcc agcgtgcaga cctgtaccca aacctatccg
     6421 tggacgccag cggttcccgt cagcgtctgc cctcagacct gtccggcacc ggccattcca
     6481 cattatcgga tcaatacggt gtgcaagttg gcgttaccgc ctgggaactg gatctgtttg
     6541 gccgggtacg cagcctgaaa gatgcgtcgc tggaaaaata tctggccagc gaagaagccc
     6601 agcgcagcac ccaactggcg ctggttgccc aagtggcaca aagctactta accctggtta
     6661 gtgatcgcca aaacttggca atcagtcgcg aaaccgtggt cgcacaggag gactctctgg
     6721 cattgattga acgccgatac gagagcggtt tgggatccga gctggatgtg cgccaagcac
     6781 aaattgcctt gcaggaagcc cgcgccaatc tggccagctt tgaacgccag gtggatcaag
     6841 actataacgc cttagccgta ctggtcggcg ggccactgcc caccctgaaa tcggtgaact
     6901 tggacaacga tgccttggcc ctcgtcgcac cagttccgca aggcctaccc tcagcactac
     6961 tcacccagcg accggacgta caacaagccg agcatcagct gcgggccgcc aatgccaata
     7021 ttggtgccgc ccgagccgcc ttcttcccaa gcattcaact caccggcgcc tatggcacca
     7081 tgagtagcga actgtccggc ctgttcgaca gcggctccaa agcttggtca ttcatcccca
     7141 gtatttccct acccattttc tccgggggcc gtaacagggc caatctggat ctagccgaag
     7201 ttcgtcaaga tcaagccgtg gccagctacg agaaagccat acaaaccgcc tttcaggaag
     7261 tcgctaatgc gctgcaagcc cgcgacactc tggctcgcca acagcaggct caacaggcac
     7321 tagtcaccgc cactgagcag tccctgacgt tgtcacagca acgctacgaa caaggcgcag
     7381 atgattacct tgcggtgttg gacgcccaac gcactctgct ctcggcccgc caacagctag
     7441 tgacgctaaa actgcagcag ttgaccaacg aaaccgagct ttaccgcgcc ttgggtggtg
     7501 gctgggatgc gcaacagccc acccatgtgg ccagccgcta aggatccaaa ctcgagtaag
     7561 gatctccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat
     7621 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc
     7681 tttctgcgtt tatacctagg gtacgggttt tgctgcccgc aaacgggctg ttctggtgtt
     7741 gctagtttgt tatcagaatc gcagatccgg cttcagccgg tttgccggct gaaagcgcta
     7801 tttcttccag aattgccatg attttttccc cacgggaggc gtcactggct cccgtgttgt
     7861 cggcagcttt gattcgataa gcagcatcgc ctgtttcagg ctgtctatgt gtgactgttg
     7921 agctgtaaca agttgtctca ggtgttcaat ttcatgttct agttgctttg ttttactggt
     7981 ttcacctgtt ctattaggtg ttacatgctg ttcatctgtt acattgtcga tctgttcatg
     8041 gtgaacagct ttgaatgcac caaaaactcg taaaagctct gatgtatcta tcttttttac
     8101 accgttttca tctgtgcata tggacagttt tccctttgat atgtaacggt gaacagttgt
     8161 tctacttttg tttgttagtc ttgatgcttc actgatagat acaagagcca taagaacctc
     8221 agatccttcc gtatttagcc agtatgttct ctagtgtggt tcgttgtttt tgcgtgagcc
     8281 atgagaacga accattgaga tcatacttac tttgcatgtc actcaaaaat tttgcctcaa
     8341 aactggtgag ctgaattttt gcagttaaag catcgtgtag tgtttttctt agtccgttat
     8401 gtaggtagga atctgatgta atggttgttg gtattttgtc accattcatt tttatctggt
     8461 tgttctcaag ttcggttacg agatccattt gtctatctag ttcaacttgg aaaatcaacg
     8521 tatcagtcgg gcggcctcgc ttatcaacca ccaatttcat attgctgtaa gtgtttaaat
     8581 ctttacttat tggtttcaaa acccattggt taagcctttt aaactcatgg tagttatttt
     8641 caagcattaa catgaactta aattcatcaa ggctaatctc tatatttgcc ttgtgagttt
     8701 tcttttgtgt tagttctttt aataaccact cataaatcct catagagtat ttgttttcaa
     8761 aagacttaac atgttccaga ttatatttta tgaatttttt taactggaaa agataaggca
     8821 atatctcttc actaaaaact aattctaatt tttcgcttga gaacttggca tagtttgtcc
     8881 actggaaaat ctcaaagcct ttaaccaaag gattcctgat ttccacagtt ctcgtcatca
     8941 gctctctggt tgctttagct aatacaccat aagcattttc cctactgatg ttcatcatct
     9001 gagcgtattg gttataagtg aacgataccg tccgttcttt ccttgtaggg ttttcaatcg
     9061 tggggttgag tagtgccaca cagcataaaa ttagcttggt ttcatgctcc gttaagtcat
     9121 agcgactaat cgctagttca tttgctttga aaacaactaa ttcagacata catctcaatt
     9181 ggtctaggtg attttaatca ctataccaat tgagatgggc tagtcaatga taattactag
     9241 tccttttccc gggtgatctg ggtatctgta aattctgcta gacctttgct ggaaaacttg
     9301 taaattctgc tagaccctct gtaaattccg ctagaccttt gtgtgttttt tttgtttata
     9361 ttcaagtggt tataatttat agaataaaga aagaataaaa aaagataaaa agaatagatc
     9421 ccagccctgt gtataactca ctactttagt cagttccgca gtattacaaa aggatgtcgc
     9481 aaacgctgtt tgctcctcta caaaacagac cttaaaaccc taaaggctta agtagcaccc
     9541 tcgcaagctc gggcaaatcg ctgaatattc cttttgtctc cgaccatcag gcacctgagt
     9601 cgctgtcttt ttcgtgacat tcagttcgct gcgctcacgg ctctggcagt gaatgggggt
     9661 aaatggcact acaggcgcct tttatggatt catgcaagga aactacccat aatacaagaa
     9721 aagcccgtca cgggcttctc agggcgtttt atggcgggtc tgctatgtgg tgctatctga
     9781 ctttttgctg ttcagcagtt cctgccctct gattttccag tctgaccact tcggattatc
     9841 ccgtgacagg tcattcagac tggctaatgc acccagtaag gcagcggtat catcaacagg
     9901 cttacccgtc ttactgtccc tagtgcttgg attctcacca ataaaaaacg cccggcggca
     9961 accgagcgtt ctgaacaaat ccagatggag ttctgaggtc attactggat ctatcaacag
    10021 gagtccaagc gagctcgata tcaaattacg ccccgccctg ccactcatcg cagtactgtt
    10081 gtaattcatt aagcattctg ccgacatgga agccatcaca aacggcatga tgaacctgaa
    10141 tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg
    10201 gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg
    10261 gattggctga gacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt
    10321 caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt
    10381 attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt
    10441 gaacactatc ccatatcacc agctcaccgt ctttcattgc catacgaaat tccggatgag
    10501 cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc ttatttttct
    10561 ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag gtacattgag
    10621 caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata tcaacggtgg
    10681 tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat ctcgataact
    10741 caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa cctcttacgt
    10801 gccgatcaac gtctcatttt cgccagatat c
//
