LOCUS       sequence-167838-        4011 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing HERPUD2 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4011)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4011)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_HERPUD2_p.R285Q
FEATURES             Location/Qualifiers
     source          1..4011
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1411..1510)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1527..1546)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1528..1546)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1551..1567)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1551..1567)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1998..2789
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2882..3470
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3371..3390
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3624..3641
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3800..3827)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3919..4005)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcat ggaccaaagt gggatggaga ttcctgtgac cctcatcatt aaagcaccga
      241 atcagaaata cagtgaccag actattagct gcttcttgaa ctggaccgtg gggaaactaa
      301 aaacgcatct atctaacgtt taccctagca aaccattgac gaaggatcag agattggtgt
      361 attcgggcag actgcttccc gatcatctgc agctgaaaga cattctcaga aaacaagatg
      421 agtatcatat ggttcatcta gtatgtactt ctcggactcc tcccagttct ccaaaatcca
      481 gcaccaatag agaaagtcat gaagcattga catccagcag caattctagt tcagatcatt
      541 caggatcaac aactccatca tctggtcaag aaaccttgtc tttagctgtg ggttcttcct
      601 cagaaggatt gaggcagcgt acccttccac aagcacaaac tgaccaagca cagagtcacc
      661 agtttccata tgtaatgcaa ggaaatgtag acaaccaatt tcctgggcaa gctgctccac
      721 ctggattccc agtgtatccc gcgtttagcc cactgcagat gctatggtgg caacagatgt
      781 atgctcatca gtattatatg cagtatcaag ctgcagtttc agctcaggcc acatcaaatg
      841 tcaacccaac ccagcctact acttcacagc ctctaaattt ggcacatgtt cctggagaag
      901 aacccccacc agctccaaac ctagtggccc aagaaaatcg acccatgaat gagaatgttc
      961 aaatgaatgc acagggaggt ccagtactaa atgaagaaga cttcaatcga gactggctag
     1021 actggatgta cacgttctca caagctgcga ttctccttag cattgtatac ttctattctt
     1081 cttttagtcg gtttatcatg gtaatgggag ccatgctact ggtttattta caccaagctg
     1141 gatggtttcc ttttaggcaa gaaggaggtc atcagcaggc tcccaacaat aatgccgaag
     1201 ttaacaatga tgggcaaaat gcaaacaact tggaacttga agaaatggag cgtcttatgg
     1261 atgatgggct tgaagatgag agtggagaag atggaggtga agatgccagt gcaattcaaa
     1321 ggcctggatt aatggcttca gcttggtctt tcatcaccac cttctttact tcactaatac
     1381 cagaggggcc tccccaggtt gccaattgat tgccaacttt cttgtacaaa gttggcatta
     1441 taagaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa
     1501 tcattatttg ccatccagct gatatcccct atagtgagtc gtattacatg gtcatagctg
     1561 tttcctggca gctctggccc gtgtctcaaa atctctgatg ttacattgca caagataaaa
     1621 atatatcatc atgcctcctc tagaccagcc aggacagaaa tgcctcgact tcgctgctac
     1681 ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc cagtggacat
     1741 aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc aactggtcca
     1801 gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg
     1861 actgtttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg
     1921 tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta
     1981 aaacaaagtt aaacattatg agggaagcgg tgatcgccga agtatcgact caactatcag
     2041 aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg
     2101 gctccgcagt ggatggcggc ctgaagccac acagtgatat tgatttgctg gttacggtga
     2161 ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg
     2221 cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg
     2281 acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca
     2341 atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc
     2401 tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg
     2461 atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact
     2521 cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt
     2581 acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc
     2641 gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag
     2701 aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccac tacgtgaaag
     2761 gcgagatcac caaggtagtc ggcaaataac cctcgagcca cccatgacca aaatccctta
     2821 acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     2881 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     2941 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     3001 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     3061 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     3121 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     3181 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     3241 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     3301 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     3361 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     3421 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     3481 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     3541 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     3601 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     3661 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     3721 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tacgcgtacc gctagccagg
     3781 aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg gccttctgct tagtttgatg
     3841 cctggcagtt tatggcgggc gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt
     3901 caaatccgct cccggcggat ttgtcctact caggagagcg ttcaccgaca aacaacagat
     3961 aaaacgaaag gcccagtctt ccgactgagc ctttcgtttt atttgatgcc t
//
