LOCUS       sequence-167843-       11474 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  target  miRNA169a  for destruction in Arabidopsis thaliana.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11474)
  AUTHORS   Tang G, Yan J, Gu Y, Qiao M, Fan R, Mao Y, Tang X
  TITLE     Construction of short tandem target mimic (STTM) to block the 
            functions of plant and animal microRNAs.
  JOURNAL   Methods. 2012 Oct;58(2):118-25. doi: 10.1016/j.ymeth.2012.10.006. 
            Epub 2012 Oct 23.
  PUBMED    23098881
REFERENCE   2  (bases 1 to 11474)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ymeth.2012.10.006"; journalName: "Methods"; date: 
            "2012-10"; volume: "58"; issue: "2"; pages: "118-25"
COMMENT     Sequence Label: pFGC5941-PacI-ath-STTM169a
FEATURES             Location/Qualifiers
     source          1..11474
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..346
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    510..686
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(1248..1266)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1249..1266)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     1905..1924
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(2027..2032,2033..2049,2050..2055))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     promoter        2330..2710
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     CDS             2716..3267
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     terminator      3277..3529
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     misc_feature    3615..3639
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             4064..4858
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      4945..5533
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5687..5704
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    5719..5859
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(5785..5804)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5945..5967
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      6203..6397
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(6463..7536)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(7965..8594)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    9895..9919
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     10107..10129
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10121..10138
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10122..10138
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(10163..10870)
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     primer_bind     11049..11068
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        11049..11067
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tgagactttt caacaaaggg taatttcggg aaacctcctc ggattccatt gcccagctac
       61 ctgtcacctc atcgaaagga cagtagaaaa ggaaggtggc tcctacaaat gccatcatcg
      121 cgataaagga aaggctatca ttcaagatgc ctctgccgac agtggtccca aagatggacc
      181 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaggcaagt
      241 ggattgatgt gacatctcca ctgacataag ggatgacgca caaccccact atccttcgca
      301 agaccctccc tctatataag gaagttcatt tcatttggag aggacagccc aagctttcgg
      361 caagtcaagt tccttggctg gttgttgttg ttatggtcta atttaaatat ggtctaaaga
      421 agaagaatat cgaagttcca agctatcctc ttcaagaatt cggtacgctg aaatcaccag
      481 tctctctcta caaatctatc tctctctatt ttctccataa ataatgtgtg agtagtttcc
      541 cgataaggga aattagggtt cttatagggt ttcgctcatg tgttgagcat ataagaaacc
      601 cttagtatgt atttgtattt gtaaaatact tctatcaata aaatttctaa ttcctaaaac
      661 caaaatccag tactaaaatc cagatctcct aaagtcccta tagatctttg tcgtgaatat
      721 aaaccagaca cgagacgact agacctggag cccagacgcc gttcgaagct agaagtaccg
      781 cttaggcagg aggccgttag ggaaaagatg ctaaggcagg gttggttacg ttgactcccc
      841 cgtaggtttg gtttaaatat gatgaagtgg acggaaggaa ggaggaagac aaggaaggat
      901 aaggttgcag gccctgtgca aggtaagaag atggaaattt gatagaggta cgctactata
      961 cttatactat acgctaaggg aatgattgta tttataccct atacccccta ataacccctt
     1021 atcaatttaa gaaataatcc gcataagccc ccgcttaaaa attggtatca gagccatgaa
     1081 taggtctatg accaaaactc aagaggataa aacctcacca aaatacgaaa gagtccttaa
     1141 ctctaaagat aaaagatctt tcaagatcaa aactagttcc ctcacaccgg tgacggggat
     1201 cgcatgcgac ctctagacca tcacattgga gcttcagctg ctcgagttct atagtgtcac
     1261 ctaaatcgta tgtgtatgat acataaggtt atgtattaat tgtagccgcg ttcttacgtc
     1321 gacgtaccaa ttacgccccg ccctgtcact catcgcagta ctgttgtaat tcattaagca
     1381 ttctgccgac atggaagcca tcacaaacgg catgatgaac ctgaatcgcc agcggcatca
     1441 gcaccttgtc gccttgcgta taatatttgc ccatggtgaa aacgggggcg aagaagttgt
     1501 ccatattggc cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgagacga
     1561 aaaacatatt ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca
     1621 catcttgcga atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagcg
     1681 atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata
     1741 ttaccagctc accgtctttc attgccatac ggaactccgg atgagcattc atcaggcggg
     1801 caagaatgtg aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa
     1861 aggccgtaat atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg
     1921 cctcaaaatg ttctttacga tgccattggg gtatatcaac ggtggtatat ccagtgattt
     1981 ctttctccat tttagcttcc ttagctcctg aaaatctcga taactcatta aagcttatcg
     2041 atgataagct gtcaaacatg agaatttagc ccgcctaatg agcgggcttt ttgttgtgat
     2101 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     2161 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttcccccct
     2221 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     2281 tttcttaatt aactctctag actcacctag gatccatgaa ttctgagatt tttcaaatca
     2341 gtgcgcaaga cgtgacgtaa gtatccgagt cagtttttat ttttctacta atttggtcgt
     2401 ttatttcggc gtgtaggaca tggcaaccgg gcctgaattt cgcgggtatt ctgtttctat
     2461 tccaactttt tcttgatccg cagccattaa cgacttttga atagatacgc tgacacgcca
     2521 agcctcgcta gtcaaaagtg taccaaacaa cgctttacag caagaacgga atgcgcgtga
     2581 cgctcgcggt gacgccattt cgccttttca gaaatggata aatagccttg cttcctatta
     2641 tatcttccca aattaccaat acattacact agcatctgaa tttcataacc aatctcgata
     2701 caccaaatcg aatcgatgag cccagaacga cgcccggccg acatccgccg tgccaccgag
     2761 gcggacatgc cggcggtctg caccatcgtc aaccactaca tcgagacaag cacggtcaac
     2821 ttccgtaccg agccgcagga accgcaggag tggacggacg acctcgtccg tctgcgggag
     2881 cgctatccct ggctcgtcgc cgaggtggac ggcgaggtcg ccggcatcgc ctacgcgggc
     2941 ccctggaagg cacgcaacgc ctacgactgg acggccgagt cgaccgtgta cgtctccccc
     3001 cgccaccagc ggacgggact gggctccacg ctctacaccc acctgctgaa gtccctggag
     3061 gcacagggct tcaagagcgt ggtcgctgtc atcgggctgc ccaacgaccc gagcgtgcgc
     3121 atgcacgagg cgctcggata tgccccccgc ggcatgctgc gggcggccgg cttcaagcac
     3181 gggaactggc atgacgtggg tttctggcag ctggacttca gcctgccggt accgccccgt
     3241 ccggtcctgc ccgtcaccga gatttgactg cagaatcttg gactcccatg ttggcaaagg
     3301 caaccaaaca aacaatgaat gatccgctcc tgcatatggg gcggtttgag tatttcaact
     3361 gccatttggg ctgaattgaa gacatgctcc tgtcagaaat tccgtgatct tactcaatat
     3421 tcagtaatct cggccaatat cctaaatgtg cgtggcttta tctgtctttg tattgtttca
     3481 tcaattcatg taacgtttgc ttttcttatg aattttcaaa taaattatca gatccgagct
     3541 cgaattaatt cggcgttaat tcagtacatt aaaaacgtcc gcaatgtgtt attaagttgt
     3601 ctaagcgtca atttgtttac accacaatat atcctgccac cagccagcca acagctcccc
     3661 gaccggcagc tcggcacaaa atcaccactc gatacaggca gcccatcagt ccgggacggc
     3721 gtcagcggga gagccgttgt aaggcggcag actttgctca tgttaccgat gctattcgga
     3781 agaacggcaa ctaagctgcc gggtttgaaa cacggatgat ctcgcggagg gtagcatgtt
     3841 gattgtaacg atgacagagc gttgctgcct gtgatcaccg cggtttcaaa atcggctccg
     3901 tcgatactat gttatacgcc aactttgaaa acaactttga aaaagctgtt ttctggtatt
     3961 taaggtttta gaatgcaagg aacagtgaat tggagttcgt cttgttataa ttagcttctt
     4021 ggggtatctt taaatactgt agaaaagagg aaggaaataa taaatggcta aaatgagaat
     4081 atcaccggaa ttgaaaaaac tgatcgaaaa ataccgctgc gtaaaagata cggaaggaat
     4141 gtctcctgct aaggtatata agctggtggg agaaaatgaa aacctatatt taaaaatgac
     4201 ggacagccgg tataaaggga ccacctatga tgtggaacgg gaaaaggaca tgatgctatg
     4261 gctggaagga aagctgcctg ttccaaaggt cctgcacttt gaacggcatg atggctggag
     4321 caatctgctc atgagtgagg ccgatggcgt cctttgctcg gaagagtatg aagatgaaca
     4381 aagccctgaa aagattatcg agctgtatgc ggagtgcatc aggctctttc actccatcga
     4441 catatcggat tgtccctata cgaatagctt agacagccgc ttagccgaat tggattactt
     4501 actgaataac gatctggccg atgtggattg cgaaaactgg gaagaagaca ctccatttaa
     4561 agatccgcgc gagctgtatg attttttaaa gacggaaaag cccgaagagg aacttgtctt
     4621 ttcccacggc gacctgggag acagcaacat ctttgtgaaa gatggcaaag taagtggctt
     4681 tattgatctt gggagaagcg gcagggcgga caagtggtat gacattgcct tctgcgtccg
     4741 gtcgatcagg gaggatatcg gggaagaaca gtatgtcgag ctattttttg acttactggg
     4801 gatcaagcct gattgggaga aaataaaata ttatatttta ctggatgaat tgttttagta
     4861 cctagaatgc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     4921 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     4981 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     5041 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     5101 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     5161 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     5221 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     5281 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     5341 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     5401 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     5461 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     5521 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     5581 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     5641 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     5701 cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
     5761 acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta
     5821 tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc
     5881 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     5941 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag
     6001 ggtgccttga tgtgggcgcc ggcggtcgag tggcgacggc gcggcttgtc cgcgccctgg
     6061 tagattgcct ggccgtaggc cagccatttt tgagcggcca gcggccgcga taggccgacg
     6121 cgaagcggcg gggcgtaggg agcgcagcga ccgaagggta ggcgcttttt gcagctcttc
     6181 ggctgtgcgc tggccagaca gttatgcaca ggccaggcgg gttttaagag ttttaataag
     6241 ttttaaagag ttttaggcgg aaaaatcgcc ttttttctct tttatatcag tcacttacat
     6301 gtgtgaccgg ttcccaatgt acggctttgg gttcccaatg tacgggttcc ggttcccaat
     6361 gtacggcttt gggttcccaa tgtacgtgct atccacagga aagagacctt ttcgaccttt
     6421 ttcccctgct agggcaattt gccctagcat ctgctccgta cattaggaac cggcggatgc
     6481 ttcgccctcg atcaggttgc ggtagcgcat gactaggatc gggccagcct gccccgcctc
     6541 ctccttcaaa tcgtactccg gcaggtcatt tgacccgatc agcttgcgca cggtgaaaca
     6601 gaacttcttg aactctccgg cgctgccact gcgttcgtag atcgtcttga acaaccatct
     6661 ggcttctgcc ttgcctgcgg cgcggcgtgc caggcggtag agaaaacggc cgatgccggg
     6721 atcgatcaaa aagtaatcgg ggtgaaccgt cagcacgtcc gggttcttgc cttctgtgat
     6781 ctcgcggtac atccaatcag ctagctcgat ctcgatgtac tccggccgcc cggtttcgct
     6841 ctttacgatc ttgtagcggc taatcaaggc ttcaccctcg gataccgtca ccaggcggcc
     6901 gttcttggcc ttcttcgtac gctgcatggc aacgtgcgtg gtgtttaacc gaatgcaggt
     6961 ttctaccagg tcgtctttct gctttccgcc atcggctcgc cggcagaact tgagtacgtc
     7021 cgcaacgtgt ggacggaaca cgcggccggg cttgtctccc ttcccttccc ggtatcggtt
     7081 catggattcg gttagatggg aaaccgccat cagtaccagg tcgtaatccc acacactggc
     7141 catgccggcc ggccctgcgg aaacctctac gtgcccgtct ggaagctcgt agcggatcac
     7201 ctcgccagct cgtcggtcac gcttcgacag acggaaaacg gccacgtcca tgatgctgcg
     7261 actatcgcgg gtgcccacgt catagagcat cggaacgaaa aaatctggtt gctcgtcgcc
     7321 cttgggcggc ttcctaatcg acggcgcacc ggctgccggc ggttgccggg attctttgcg
     7381 gattcgatca gcggccgctt gccacgattc accggggcgt gcttctgcct cgatgcgttg
     7441 ccgctgggcg gcctgcgcgg ccttcaactt ctccaccagg tcatcaccca gcgccgcgcc
     7501 gatttgtacc gggccggatg gtttgcgacc gctcacgccg attcctcggg cttgggggtt
     7561 ccagtgccat tgcagggccg gcaggcaacc cagccgctta cgcctggcca accgcccgtt
     7621 cctccacaca tggggcattc cacggcgtcg gtgcctggtt gttcttgatt ttccatgccg
     7681 cctcctttag ccgctaaaat tcatctactc atttattcat ttgctcattt actctggtag
     7741 ctgcgcgatg tattcagata gcagctcggt aatggtcttg ccttggcgta ccgcgtacat
     7801 cttcagcttg gtgtgatcct ccgccggcaa ctgaaagttg acccgcttca tggctggcgt
     7861 gtctgccagg ctggccaacg ttgcagcctt gctgctgcgt gcgctcggac ggccggcact
     7921 tagcgtgttt gtgcttttgc tcattttctc tttacctcat taactcaaat gagttttgat
     7981 ttaatttcag cggccagcgc ctggacctcg cgggcagcgt cgccctcggg ttctgattca
     8041 agaacggttg tgccggcggc ggcagtgcct gggtagctca cgcgctgcgt gatacgggac
     8101 tcaagaatgg gcagctcgta cccggccagc gcctcggcaa cctcaccgcc gatgcgcgtg
     8161 cctttgatcg cccgcgacac gacaaaggcc gcttgtagcc ttccatccgt gacctcaatg
     8221 cgctgcttaa ccagctccac caggtcggcg gtggcccata tgtcgtaagg gcttggctgc
     8281 accggaatca gcacgaagtc ggctgccttg atcgcggaca cagccaagtc cgccgcctgg
     8341 ggcgctccgt cgatcactac gaagtcgcgc cggccgatgg ccttcacgtc gcggtcaatc
     8401 gtcgggcggt cgatgccgac aacggttagc ggttgatctt cccgcacggc cgcccaatcg
     8461 cgggcactgc cctggggatc ggaatcgact aacagaacat cggccccggc gagttgcagg
     8521 gcgcgggcta gatgggttgc gatggtcgtc ttgcctgacc cgcctttctg gttaagtaca
     8581 gcgataacct tcatgcgttc cccttgcgta tttgtttatt tactcatcgc atcatatacg
     8641 cagcgaccgc atgacgcaag ctgttttact caaatacaca tcaccttttt agacggcggc
     8701 gctcggtttc ttcagcggcc aagctggccg gccaggccgc cagcttggca tcagacaaac
     8761 cggccaggat ttcatgcagc cgcacggttg agacgtgcgc gggcggctcg aacacgtacc
     8821 cggccgcgat catctccgcc tcgatctctt cggtaatgaa aaacggttcg tcctggccgt
     8881 cctggtgcgg tttcatgctt gttcctcttg gcgttcattc tcggcggccg ccagggcgtc
     8941 ggcctcggtc aatgcgtcct cacggaaggc accgcgccgc ctggcctcgg tgggcgtcac
     9001 ttcctcgctg cgctcaagtg cgcggtacag ggtcgagcga tgcacgccaa gcagtgcagc
     9061 cgcctctttc acggtgcggc cttcctggtc gatcagctcg cgggcgtgcg cgatctgtgc
     9121 cggggtgagg gtagggcggg ggccaaactt cacgcctcgg gccttggcgg cctcgcgccc
     9181 gctccgggtg cggtcgatga ttagggaacg ctcgaactcg gcaatgccgg cgaacacggt
     9241 caacaccatg cggccggccg gcgtggtggt gtcggcccac ggctctgcca ggctacgcag
     9301 gcccgcgccg gcctcctgga tgcgctcggc aatgtccagt aggtcgcggg tgctgcgggc
     9361 caggcggtct agcctggtca ctgtcacaac gtcgccaggg cgtaggtggt caagcatcct
     9421 ggccagctcc gggcggtcgc gcctggtgcc ggtgatcttc tcggaaaaca gcttggtgca
     9481 gccggccgcg tgcagttcgg cccgttggtt ggtcaagtcc tggtcgtcgg tgctgacgcg
     9541 ggcatagccc agcaggccag cggcggcgct cttgttcatg gcgtaatgtc tccggttcta
     9601 gtcgcaagta ttctacttta tgcgactaaa acacgcgaca agaaaacgcc aggaaaaggg
     9661 cagggcggca gcctgtcgcg taacttagga cttgtgcgac atgtcgtttt cagaagacgg
     9721 ctgcactgaa cgtcagaagc cgactgcact atagcagcgg aggggttgga tcaaagtact
     9781 ttgatcccga ggggaaccct gtggttggca tgcacataca aatggacgaa cggataaacc
     9841 ttttcacgcc cttttaaata tccgattatt ctaataaacg ctcttttctc ttaggtttac
     9901 ccgccaatat atcctgtcaa acactgatag tttaaactga aggcgggaaa cgacaatctg
     9961 atccaagctc aagctgctct agcattcgcc attcaggctg cgcaactgtt gggaagggcg
    10021 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
    10081 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtgc
    10141 caagcttgca tgcctgcagg tcctgctgag cctcgacatg ttgtcgcaaa attcgccctg
    10201 gacccgccca acgatttgtc gtcactgtca aggtttgacc tgcacttcat ttggggccca
    10261 catacaccaa aaaaatgctg cataattctc ggggcagcaa gtcggttacc cggccgccgt
    10321 gctggaccgg gttgaatggt gcccgtaact ttcggtagag cggacggcca atactcaact
    10381 tcaaggaatc tcacccatgc gcgccggcgg ggaaccggag ttcccttcag tgagcgttat
    10441 tagttcgccg ctcggtgtgt cgtagatact agcccctggg gcacttttga aatttgaata
    10501 agatttatgt aatcagtctt ttaggtttga ccggttctgc cgcttttttt aaaattggat
    10561 ttgtaataat aaaacgcaat tgtttgttat tgtggcgctc tatcatagat gtcgctataa
    10621 acctattcag cacaatatat tgttttcatt ttaatattgt acatataagt agtagggtac
    10681 aatcagtaaa ttgaacggag aatattattc ataaaaatac gatagtaacg ggtgatatat
    10741 tcattagaat gaaccgaaac cggcggtaag gatctgagct acacatgctc aggtttttta
    10801 caacgtgcac aacagaattg aaagcaaata tcatgcgatc ataggcgtct cgcatatctc
    10861 attaaagcag gactctaggg actagtcccg ggtcttaatt aagtttgcaa gcagcagatt
    10921 acgcgcagaa aaaaaggatc tcaaggagat cctttggatc acaaaaaaaa agcccgctca
    10981 ttaggcgggt taaattcccg ggtactgcag ccgattcatt aatgcaggtt aacctggctt
    11041 atcgaaatta atacgactca ctatagggag accggcagat ctgatcccta ctccaaaaat
    11101 gtcaaagata cagtctcaga agaccaaagg gctattgaga cttttcaaca aagggtaatt
    11161 tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagtg
    11221 gaaaaggaag gtggctccta caaatgccat cattgcgata aaggaaaggc tatcattcaa
    11281 gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggga
    11341 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgacat ctccactgac
    11401 gtaagggatg acgcacaatc ccgcccctac tccaaaaatg tcaaagatac tgtctcagaa
    11461 gaccaaaggg ctat
//
