LOCUS       sequence-167848-         770 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  target  miRNA780.1  for destruction in Arabidopsis thaliana.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Tang G, Yan J, Gu Y, Qiao M, Fan R, Mao Y, Tang X
  TITLE     Construction of short tandem target mimic (STTM) to block the 
            functions of plant and animal microRNAs.
  JOURNAL   Methods. 2012 Oct;58(2):118-25. doi: 10.1016/j.ymeth.2012.10.006. 
            Epub 2012 Oct 23.
  PUBMED    23098881
REFERENCE   2  (bases 1 to 770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ymeth.2012.10.006"; journalName: "Methods"; date: 
            "2012-10"; volume: "58"; issue: "2"; pages: "118-25"
COMMENT     Sequence Label: pFGC5941-PacI-ath-STTM780.1
FEATURES             Location/Qualifiers
     source          1..770
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     115..134
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(276..281,282..298,299..304)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(407..426)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 aatctcagaa ttcatggatc ctaggtgagt ctagagagtt aattaagaaa ggcggacagg
       61 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc aggggggaaa
      121 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
      181 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg atcacaacca
      241 aaagcccgct cattaggcgg gctaaattct catgtttgac agcttatcat cgataagctt
      301 taatgagtta tcgagatttt caggagctaa ggaagctaaa atggagaaag aaatcactgg
      361 atataccacc gttgatgtat cccaatggca tcgtaaagaa cattttgagg catttcagtc
      421 agttgctcaa tgtacctata accagaccgt tcagctggat attacggcct ttttaaagac
      481 cgtaaagaaa aataagcaca agttttatcc ggcctttatt cacattcttg cccgcctgat
      541 gaatgctcat ccggagttcc gtatggcaat gaaagacggt gagctggtaa tatgggacag
      601 tgttcaccct tgttacaccg ttttccatga gcaaactgaa acgttttcat cgctctggag
      661 tgaataccac gacgatttcc ggcagtttct acacatatat tcgcaagatg tggcgtgtta
      721 cggtgaaaac ctggcctatt tccctaaagg gtttattgag aatatgtttt
//
