LOCUS       sequence-167850-         750 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Mj aaRS for sulfotyrosine and Mj tRNA for decoding UAG 
            codons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Schwessinger B, Li X, Ellinghaus TL, Chan LJ, Wei T, Joe A, Thomas 
            N, Pruitt R, Adams PD, Chern MS, Petzold CJ, Liu CC, Ronald PC
  TITLE     A second-generation expression system for tyrosine-sulfated proteins
            and its application in crop protection.
  JOURNAL   Integr Biol (Camb). 2016 Apr 18;8(4):542-5. doi: 10.1039/c5ib00232j.
            Epub 2015 Nov 27.
  PUBMED    26611838
REFERENCE   2  (bases 1 to 750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1039/c5ib00232j"; journalName: "Integr Biol (Camb)"; date: 
            "2016-04-18- 18"; volume: "8"; issue: "4"; pages: "542-5"
COMMENT     Sequence Label: pUltra-sY
FEATURES             Location/Qualifiers
     source          1..750
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     106..123
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
ORIGIN
        1 tcggcgctac ggcgtttcac ttctgagttc ggcatggggt caggtgggac caccgcgcta
       61 ctgccgccag gcaaattctg ttttatcaga ccgcttctgc gttctgattt aatctgtatc
      121 aggctgaaaa tcttctctca tccgccaaaa cagccaagct ggagaccgtt taaacgcggc
      181 cgcttataat ctctttctaa ttggctctaa aatctttata agttcttcag ctacagcatt
      241 ttttaaatcc attggatgca attccttatt tttaaataaa ctctctaact cctcatagct
      301 attaactgtc aaatctccac caaatttttc tggccttttt atggttaaag gatattcaag
      361 gaagtattta gctatctcca ttattggatt tccttcaaca actccagctg ggcagtatgc
      421 tttctttatc ttagccctaa tctcttctgg agagtcatca acagctataa aattcccttt
      481 tgaagaactc atctttcctt ctccatccaa acccgttaag acagggttgt gaatacaaac
      541 aacctttttt ggtaaaagct cccttgctaa catgtgtatt tttctctgct ccatccctcc
      601 aactgcaaca tcaacgccct tataatgaca accattaacc tgcattattg gatagataac
      661 ttcagcaacc tttggatttt catcctctct tgctataagt tccatactcc ttcttgctct
      721 ttttaaggta gtttttaaag ccaatctata
//
