LOCUS       sequence-167851-        1061 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Hsp27 expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1061)
  TITLE     Moens lab Hsp27 plasmids
REFERENCE   2  (bases 1 to 1061)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pFLAG-CMV2-Hsp27-S15D/S82D
FEATURES             Location/Qualifiers
     source          1..1061
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(880..903)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(937..1036)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(966..986)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 tgtcttccca acttgcccct tgctccatac cacccccctc caccccataa tattatagaa
       61 ggacacctag tcagacaaaa tgatgcaact taattttatt aggacaaggc tggtgggcac
      121 tggagtggca acttccaggg ccaggagagg cactggggag gggtcacagg gatgccaccc
      181 gggatccggg ctaaggcttt acttggcggc agtctcatcg gattttgcag cttctgggcc
      241 cccaagctgg gcccgcgact cgaaggtgac tgggatggtg atctcgttgg actgcgtggc
      301 tagcttgggc atgggggcct ccacggtcag tgtgccctca ggggacaggg aggaggaaac
      361 ttgggtgggg tccacaccgg ggggcagcgt gtatttccgc gtgaagcacc gggagatgta
      421 gccatgctcg tcctgccgct cctcgtgctt gccggtgatc tccaccacgc catccttggt
      481 cttgaccgtc agctcgtccg gggcgaagtg gttgacatcc agggacacgc gccagcggtc
      541 cgcagtgtgc cggatctccg agaccccgct atcgagttgc cggctgagcg cgcggctgta
      601 ggcgggcgcg gccactgcgg ggctctcgat ggcggcgggg ggcagggggc gcacgtagcc
      661 tggccagctg ctgccgccta accactgcga ccactcctcc ggcagccggg gcagcccgaa
      721 ggcctggtcg aagaggcggc tatgcgggta ccagtcgcgg aaggggtccc aatcggggcc
      781 ccgcaggagc gagaagggga cgcggcgctc ggtcatgctg gatcctctag agtcgactgg
      841 taccgatatc agatctatcg atgaattcgc ggccgcaagc ttgtcgtcat cgtctttgta
      901 gtccatggta gatcaattct gacggttcac taaacgagct ctgcttatat agacctccca
      961 ccgtacacgc ctaccgccca tttgcgtcaa cggggcgggg ttattacgac attttggaaa
     1021 gtcccgttga ttttggtgcc aaaacaaact cccattgacg t
//
