LOCUS       sequence-167870-        3911 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing MAGEC2 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3911)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 3911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_MAGEC2_p.G74V
FEATURES             Location/Qualifiers
     source          1..3911
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1311..1410)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1427..1446)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1428..1446)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1451..1467)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1451..1467)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1898..2689
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2782..3370
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3271..3290
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3524..3541
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3700..3727)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3819..3905)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catgcctccc gttccaggcg ttccattccg caacgttgac aacgactccc
      241 cgacctcagt tgagttagaa gactgggtag atgcacagca tcccacagat gaggaagagg
      301 aggaagcctc ctccgcctct tccactttgt acttagtatt ttccccctct tctttctcca
      361 catcctcttc tctgattctt ggtggtcctg aggaggagga ggtgccctct gttgtgatac
      421 caaatcttac cgagagcatt cccagtagtc ctccacaggg tcctccacag ggtccttccc
      481 agagtcctct gagctcctgc tgctcctctt tttcatggag ctcattcagt gaggagtcca
      541 gcagccagaa aggggaggat acaggcacct gtcagggcct gccagacagt gagtcctctt
      601 tcacatatac actagatgaa aaggtggccg agttagtgga gttcctgctc ctcaaatacg
      661 aagcagagga gcctgtaaca gaggcagaga tgctgatgat tgtcatcaag tacaaagatt
      721 actttcctgt gatactcaag agagcccgtg agttcatgga gcttcttttt ggccttgccc
      781 tgatagaagt gggccctgac cacttctgtg tgtttgcaaa cacagtaggc ctcaccgatg
      841 agggtagtga tgatgagggc atgcccgaga acagcctcct gattattatt ctgagtgtga
      901 tcttcataaa gggcaactgt gcctctgagg aggtcatctg ggaagtgctg aatgcagtag
      961 gggtatatgc tgggagggag cacttcgtct atggggagcc tagggagctc ctcactaaag
     1021 tttgggtgca gggacattac ctggagtatc gggaggtgcc ccacagttct cctccatatt
     1081 atgaattcct gtggggtcca agagcccatt cagaaagcat caagaagaaa gtactagagt
     1141 ttttagccaa gctgaacaac actgttccta gttcctttcc atcctggtac aaggatgctt
     1201 tgaaagatgt ggaagagaga gtccaggcca caattgatac cgcagatgat gccactgtca
     1261 tggccagtga aagcctcagt gtcatgtcca gcaacgtctc cttttcgagt gcccaacttt
     1321 cttgtacaaa gttggcatta taagaaagca ttgcttatca atttgttgca acgaacaggt
     1381 cactatcagt caaaataaaa tcattatttg ccatccagct gatatcccct atagtgagtc
     1441 gtattacatg gtcatagctg tttcctggca gctctggccc gtgtctcaaa atctctgatg
     1501 ttacattgca caagataaaa atatatcatc atgcctcctc tagaccagcc aggacagaaa
     1561 tgcctcgact tcgctgctac ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa
     1621 ggcacgaacc cagtggacat aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat
     1681 gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc
     1741 ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa
     1801 gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac
     1861 gcagcagggc agtcgcccta aaacaaagtt aaacattatg agggaagcgg tgatcgccga
     1921 agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt
     1981 gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat
     2041 tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa
     2101 cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt
     2161 caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca
     2221 atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga
     2281 cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc
     2341 agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga
     2401 aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct
     2461 tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc
     2521 tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag
     2581 acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga
     2641 atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataac cctcgagcca
     2701 cccatgacca aaatccctta acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt
     2761 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     2821 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     2881 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     2941 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     3001 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     3061 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     3121 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     3181 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     3241 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     3301 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     3361 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     3421 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     3481 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     3541 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     3601 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     3661 tacgcgtacc gctagccagg aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg
     3721 gccttctgct tagtttgatg cctggcagtt tatggcgggc gtcctgcccg ccaccctccg
     3781 ggccgttgct tcacaacgtt caaatccgct cccggcggat ttgtcctact caggagagcg
     3841 ttcaccgaca aacaacagat aaaacgaaag gcccagtctt ccgactgagc ctttcgtttt
     3901 atttgatgcc t
//
