LOCUS       sequence-167883-        1035 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Hsp27 expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1035)
  TITLE     Moens lab Hsp27 plasmids
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pFLAG-CMV2-Hsp27-S15D/S78D/S82D
FEATURES             Location/Qualifiers
     source          1..1035
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(879..902)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(936..1035)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(965..985)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 gtcttcccaa cttgcccctt gctccatacc acccccctcc accccataat attatagaag
       61 gacacctagt cagacaaaat gatgcaactt aattttatta ggacaaggct ggtgggcact
      121 ggagtggcaa cttccagggc caggagaggc actggggagg ggtcacaggg atgccacccg
      181 ggatccgggc taaggcttta cttggcggca gtctcatcgg attttgcagc ttctgggccc
      241 ccaagctggg cccgcgactc gaaggtgact gggatggtga tctcgttgga ctgcgtggct
      301 agcttgggca tgggggcctc cacggtcagt gtgccctcag gggacaggga ggaggaaact
      361 tgggtggggt ccacaccggg gggcagcgtg tatttccgcg tgaagcaccg ggagatgtag
      421 ccatgctcgt cctgccgctc ctcgtgcttg ccggtgatct ccaccacgcc atccttggtc
      481 ttgaccgtca gctcgtccgg ggcgaagtgg ttgacatcca gggacacgcg ccagcggtcc
      541 gcagtgtgcc ggatctccga gaccccgcta tcgagttgcc gatcgagcgc gcggctgtag
      601 gcgggcgcgg ccactgcggg gctctcgatg gcggcggggg gcagggggcg cacgtagcct
      661 ggccagctgc tgccgcctaa ccactgcgac cactcctccg gcagccgggg cagcccgaag
      721 gcctggtcga agaggcggct atgcgggtac cagtcgcgga aggggtccca atcggggccc
      781 cgcaggagcg agaaggggac gcggcgctcg gtcatgctgg atcctctaga gtcgactggt
      841 accgatatca gatctatcga tgaattcgcg gccgcaagct tgtcgtcatc gtctttgtag
      901 tccatggtag atcaattctg acggttcact aaacgagctc tgcttatata gacctcccac
      961 cgtacacgcc taccgcccat ttgcgtcaac ggggcggggt tattacgaca ttttggaaag
     1021 tcccgttgat tttgg
//
