LOCUS       sequence-16789-e        1397 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1999
REFERENCE   2  (bases 1 to 1397)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: L4458
FEATURES             Location/Qualifiers
     source          1..1397
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             362..382
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(425..453)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 aatnnnntgn ggtcgactct agaggatccc caattcttga gacttccact atcttctctg
       61 gcaccccccn tnacgcgtag gcgtctctcg cttcgtgtat tcccgggaag ccgggttccc
      121 gtctctcccg ccgctgccgc tgccgcacac agctttacac ctcgtagaat ccccaaagag
      181 gggcgtggct tgcgggtgcc aacatcctcc tgccgaggaa gaagcaggca ctcatcactc
      241 gcatcatcaa cctcgggatt ggccaaagga cccaaaggta tgtttcgaat gatactaaca
      301 taacatagaa cattttcagg aggacccttg gagggtaccg agctcagaaa aaatgactgc
      361 tccaaagaag aagcgtaagg taccggtaga aaaaatgagt aaaggagaag aacttttcac
      421 tggagttgtc ccaattcttg ttgaattaga tggtgatgtt aatgggcaca aattttctgt
      481 cagtggagag ggtgaaggtg atgcaacata cggaaaactt acccttaaat ttatttgcac
      541 tactggaaaa ctacctgttc catgggtaag tttaaacata tatatactaa ctaaccctga
      601 ttatttaaat tttcagccaa cacttgtcac tactttctgt tatggtgttc aatgcttctc
      661 gagataccca gatcatatga aacggcatga ctttttcaag agtgccatgc ccgaaggtta
      721 tgtacaggaa agaactatat ttttcaaaga tgacgggaac tacaagacac gtaagtttaa
      781 acagttcggt actaactaac catacatatt taaattttca ggtgctgaag tcaagtttga
      841 aggtgatacc cttgttaata gaatcgagtt aaaaggtatt gattttaaag aagatggaaa
      901 cattcttgga cacaaattgg aatacaacta taactcacac aatgtataca tcatggcaga
      961 caaacaaaag aatggaatca aagttgtaag tttaaacatg atttactaac taactaatct
     1021 gatttaattt ncananttnc aaattanacn cacattgnag atggaagcgt ncactagcan
     1081 acnttntcac aaaatcncna ttggggangg cctgtctttt acaaaaacat nactgtccac
     1141 nanntgccct ttnaaaatcc acnaaaanna aacnntggcc ntntngattn naacccnngg
     1201 gnatacncng gngggagant tanaanacct cngnaatgga gntaacttcc nanaaaaacn
     1261 ccttnttant ttnaaatcaa ngnggcgggc cncatanant nggggnnaaa aangggcngt
     1321 nccaaaatta cgntnnccna aaaattnttt tnncgcngcg gaanaaaant naaccagntt
     1381 ccggncccnt ttaaaaa
//
