LOCUS       sequence-167897-         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yellow/red FRET biosensor for monotoring plasmamembrane 
            PtdIns(4,5)P2 levels (PIP2).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Bindels DS, Haarbosch L, van Weeren L, Postma M, Wiese KE, Mastop M,
            Aumonier S, Gotthard G, Royant A, Hink MA, Gadella TW Jr
  TITLE     mScarlet: a bright monomeric red fluorescent protein for cellular 
            imaging.
  JOURNAL   Nat Methods. 2016 Nov 21. doi: 10.1038/nmeth.4074.
  PUBMED    27869816
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Nov 21. doi: 10.1038/nmeth.4074."
COMMENT     Sequence Label: PH-PLCD1_mScarlet-H_IRES_SYFP2_PH_N1
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      106..115
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(709..808)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(738..758)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ggccctcgcc ctcgccctcg atctcgaact cgtggccgtt catggagccc tccatgtgca
       61 ccttgaaccg catgaactcc ttgatcactg cctcgccctt gctcaccatg gtggcgaccg
      121 gtggatcctt caggaagttc tgcagctcct tgaagctcat cttgttgtcc ttgtttttgt
      181 cagcttttcg caagcaggag tgaatccagt gctgtagctt ctgacgctgg tccatggagc
      241 ctgagtggtg gatgatcttg tgcagcccca gcacccagtg ctgggcatca gctggcgatg
      301 gggcgatgag gtctagtgta ttgcgctggt ccttgaagac aatggagaag cagcggtcct
      361 cgggcacatc acgggcgaac ttctccagac cctccgtgcg gtgccccatt cgcacctcct
      421 gaatgtcctc gatggagaac agctgggact ccggggtccg catgaccttg cgggactcct
      481 gccagatggt cttgcagtcc tcctgcaact tgtagaagcg ctctctcctc catgagctgg
      541 acttcacctt caggagctgg ctgcccttca gcagcgcctg tagatcctca tcatcctgta
      601 ggccgtgcag ggtcaggaag tcccggcccg agtccatgcc gtgaattcga agcttgagct
      661 cgagatctga gtccggtagc gctagcggat ctgacggttc actaaaccag ctctgcttat
      721 atagacctcc caccgtacac gcctaccgcc catttgcgtc aatggggcgg agttgttacg
      781 acattttgga aagtcccgtt gattttggtg ccaaaacaaa ctcccattga cgtcaatggg
//
