LOCUS       sequence-167901-         802 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yellow/red FRET biosensor for monotoring plasmamembrane 
            PtdIns(4,5)P2 levels (PIP2).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Bindels DS, Haarbosch L, van Weeren L, Postma M, Wiese KE, Mastop M,
            Aumonier S, Gotthard G, Royant A, Hink MA, Gadella TW Jr
  TITLE     mScarlet: a bright monomeric red fluorescent protein for cellular 
            imaging.
  JOURNAL   Nat Methods. 2016 Nov 21. doi: 10.1038/nmeth.4074.
  PUBMED    27869816
REFERENCE   2  (bases 1 to 802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Nov 21. doi: 10.1038/nmeth.4074."
COMMENT     Sequence Label: PH-PLCD1_mScarlet-H_IRES_SYFP2_PH_N1
FEATURES             Location/Qualifiers
     source          1..802
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             102..353
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     290..311
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
       61 tggagtacaa ctacaacagc cacaacgtct atatcaccgc cgacaagcag aagaacggca
      121 tcaaggccaa cttcaagatc cgccacaaca tcgaggacgg cggcgtgcag ctcgccgacc
      181 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
      241 tgagctacca gtccaagctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
      301 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtccggac
      361 tcagatctcg aggcctacag gatgatgagg atctacaggc gctgctgaag ggcagccagc
      421 tcctgaaggt gaagtccagc tcatggagga gagagcgctt ctacaagttg caggaggact
      481 gcaagaccat ctggcaggag tcccgcaagg tcatgcggac cccggagtcc cagctgttct
      541 ccatcgagga cattcaggag gtgcgaatgg ggcaccgcac ggagggtctg gagaagttcg
      601 cccgtgatgt gcccgaggac cgctgcttct ccattgtctt caaggaccag cgcaatacac
      661 tagacctcat cgccccatcg ccagctgatg cccagcactg ggtgctgggg ctgcacaaga
      721 tcatccacca ctcaggctcc atggaccagc gtcagaagct acagcactgg attcactcct
      781 gcttgcgaaa agctgacaaa aa
//
