LOCUS       sequence-167906-         718 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Green/red FRET biosensor to monitor RhoA nucleotide loading state 
            and activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 718)
  AUTHORS   Bindels DS, Haarbosch L, van Weeren L, Postma M, Wiese KE, Mastop M,
            Aumonier S, Gotthard G, Royant A, Hink MA, Gadella TW Jr
  TITLE     mScarlet: a bright monomeric red fluorescent protein for cellular 
            imaging.
  JOURNAL   Nat Methods. 2016 Nov 21. doi: 10.1038/nmeth.4074.
  PUBMED    27869816
REFERENCE   2  (bases 1 to 718)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Nov 21. doi: 10.1038/nmeth.4074."
COMMENT     Sequence Label: pTriEx-RhoA-wt_mScarlet-i_SGFP2
FEATURES             Location/Qualifiers
     source          1..718
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(486..505)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ctcgcccttg ctcacggatc cgtttgccag ctcctctgct gcctgtctct gttccttctg
       61 cttttgtgct tcagatccgt ttgccagctc ctctgctgcc tgtctctgtt ccttctgctt
      121 ttgtgcttca gatccgtttg ccagctcctc tgctgcctgt ctctgttcct tctgcttttg
      181 tgcttcctcg atgttagatc tgagtccgga cttgtacagc tcgtccatgc cgccggtgga
      241 gtggcggccc tcggagcgtt cgtactgttc caccacggtg tagtcctcgt tgtgggaggt
      301 gatgtccaac ttgcggtcga cgttgtaggc gccgggcatc tgcacgggct tcttggcctt
      361 gtaggtggtc ttgaagtccg ccaggtagcg gccgccgtcc ttcaggcgca gggccatctt
      421 aatgtcgccc ttcagcacgc cgtcctcggg gtacaaccgc tcggtggacg cttcccagcc
      481 cattgtcttc ttctgcatta cggggccgtc aggagggaag ttggtgccgc ggagcttcac
      541 cttgtagatc agggtgccgt cctccaggga ggtgtcctgg gtcacggtca cggcgccgcc
      601 gtcctcgaag ttcatcacgc gctcccactt gaagccctcg gggaaggact gcttatagta
      661 gtcggggatg tcggcggggt gcttgatgaa ggccctggag ccgtacatga actgaggg
//
