LOCUS       sequence-167908-         895 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Green/red FRET biosensor to monitor RhoA nucleotide loading state 
            and activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Bindels DS, Haarbosch L, van Weeren L, Postma M, Wiese KE, Mastop M,
            Aumonier S, Gotthard G, Royant A, Hink MA, Gadella TW Jr
  TITLE     mScarlet: a bright monomeric red fluorescent protein for cellular 
            imaging.
  JOURNAL   Nat Methods. 2016 Nov 21. doi: 10.1038/nmeth.4074.
  PUBMED    27869816
REFERENCE   2  (bases 1 to 895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Nov 21. doi: 10.1038/nmeth.4074."
COMMENT     Sequence Label: pTriEx-RhoA-wt_mScarlet-i_SGFP2
FEATURES             Location/Qualifiers
     source          1..895
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             602..634
                     /codon_start=1
                     /product="HSV (herpes simplex virus) epitope tag"
                     /label=HSV tag
                     /translation="QPELAPEDPED"
     CDS             641..664
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     primer_bind     complement(754..773)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    819..874
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(873..892)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
ORIGIN
        1 agaactggtg attgttggtg atggagcctg tggaaagaca tgcttgctca tagtcttcag
       61 caaggaccag ttcccagagg tgtatgtgcc cacagtgttt gagaactatg tggcagatat
      121 cgaggtggat ggaaagcagg tagagttggc tttgtgggac acagctgggc aggaagatta
      181 tgatcgcctg aggcccctct cctacccaga taccgatgtt atactgatgt gtttttccat
      241 cgacagccct gatagtttag aaaacatccc agaaaagtgg accccagaag tcaagcattt
      301 ctgtcccaac gtgcccatca tcctggttgg gaataagaag gatcttcgga atgatgagca
      361 cacaaggcgg gagcttgcca agatgaagca ggagccggtg aaacctgaag aaggcagaga
      421 tatggcaaac aggattggcg cttttgggta catggagtgt tcagcaaaga ccaaagatgg
      481 agtgagagag gtttttgaaa tggctacgag agctgctctg caagctagac gtgggaagaa
      541 aaaatctggg tgccttgtct tgtgaaagct tgcggccgca cagctgtata cacgtgcaag
      601 ccagccagaa ctcgccccgg aagaccccga ggatctcgag caccaccatc accatcacca
      661 tcactaagtg attaacctca ggtgcaggct gcctatcaga aggtggtggc tggtgtggcc
      721 aatgccctgg ctcacaaata ccactgagat cgatcttttt ccctctgcca aaaattatgg
      781 ggacatcatg aagccccttg agcatctgac ttctggctaa taaaggaaat ttattttcat
      841 tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga aggacatatg ggagg
//
