LOCUS       sequence-167910-         883 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  lentiviral expression vector for mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Hess GT, Fresard L, Han K, Lee CH, Li A, Cimprich KA, Montgomery SB,
            Bassik MC
  TITLE     Directed evolution using dCas9-targeted somatic hypermutation in 
            mammalian cells.
  JOURNAL   Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4038.
  PUBMED    27798611
REFERENCE   2  (bases 1 to 883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Oct 31. doi: 10.1038/nmeth.4038."
COMMENT     Sequence Label: pGH044_mCherry
FEATURES             Location/Qualifiers
     source          1..883
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             20..730
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(165..183)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     433..452
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tgtcgtgagc tagcccacca tggtgagcaa gggcgaggag gataacatgg ccatcatcaa
       61 ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat
      121 cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac
      181 caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc
      241 caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga
      301 gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca
      361 ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt
      421 cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg
      481 gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga
      541 cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct
      601 gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac
      661 catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct
      721 gtacaagtaa tctagagttg aaccgactag taagccaggt cgagacatgt ttaagggttc
      781 cggttccact aggtacaatt cgatatcaag cttatcgata atcaacctct ggattacaaa
      841 atttgtgaaa gattgactgg tattcttaac tatgttgctc ctt
//
