LOCUS       sequence-16792-e        1404 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1999
REFERENCE   2  (bases 1 to 1404)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: L453
FEATURES             Location/Qualifiers
     source          1..1404
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     42..58
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     43..59
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(827..843)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(827..843)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(875..894)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(876..894)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(901..918)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(901..917)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(910..932)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 agatacctcc taagggacaa aagctggtac cgggcccccc ctcgaggtcg acggtatcga
       61 cccntttgat atcgaattcg gaagatgggt tccatgcgcc aangttaagg atacgaaggc
      121 tcatattgat ggtctcaaga agggacaaac atatcagttc cgtgtcaagg ctgtcaataa
      181 ggaaggagct tctgatgcat tgtctactga taaggacacc aaagccaaga atccatatga
      241 tgagccagga aaaaccggaa ctccggatgt tgtcgactgg gatgctgatc gtgtctcact
      301 tgaatgggaa ccaccaaagt ctgacggagg agctccaatc actcaatacg tcattgagaa
      361 gaagggcaaa catggaagag actggcaaga atgcggaaag gtttctggag atcaaaccaa
      421 tgctgagatt cttggactca aggagggaga agagtaccag ttccgtgtga aggctgttaa
      481 caaggccgga ccgggagagg cttcagaccc aagccgaaag gttgttgcaa agccaagaaa
      541 cttgaagcca tggattgatc gtgaagcaat gaagacgatc actatcaagg taggaaacga
      601 tgtggaattc gatgttccag tacgcggaga accaccaccg aagaaggaat ggatcttcaa
      661 tgagaaacca gtcgatgatc aaaagatcag gattgaaagc gaagactaca agacccgatt
      721 tgtgctccgt ggagcaactc gcaagcatgc tggtttgtac actcttactg ctaccaacgc
      781 ttctggaagc gacaaacatt ccgttgaggt cgttgtgctc gggggggatc cactagttct
      841 agagcggccg ccaccgcggt ggagctccaa ttcgccctat agtgagtcgt attacaattc
      901 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
      961 ccttgcagca catcccccnt ttcncngctg gcgtaatagc gaanaggccn gcacngatcg
     1021 ccctttccna cagttgccca acctgaatgg cgaatgggaa cccccctgta ncggcccntn
     1081 agcgcggngg gngnggggtt acnnccaggn gacngctacc ttgcngggcc ctangcccnc
     1141 tcntttcctt tntnccttct ttccnccngt tgncggnttt ccngtaagct ntaatcgggg
     1201 ctccnttngg gttccatttt nnntttcgnc ccccccccaa ancttttnag gggnggtncc
     1261 gaggggccnc cccnaaaang tttncccttn agtggncccc ttttnaaggn acttntcaag
     1321 gaaacccccc cncgnctntt tttnnaaggn tncccccccn gnaaaagngt taaattncnt
     1381 tnaaanccct ttgggtnggn gggc
//
