LOCUS       sequence-167923-         907 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Green/red FRET biosensor for RhoA in the GTP locked constitutively 
            active state.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Bindels DS, Haarbosch L, van Weeren L, Postma M, Wiese KE, Mastop M,
            Aumonier S, Gotthard G, Royant A, Hink MA, Gadella TW Jr
  TITLE     mScarlet: a bright monomeric red fluorescent protein for cellular 
            imaging.
  JOURNAL   Nat Methods. 2016 Nov 21. doi: 10.1038/nmeth.4074.
  PUBMED    27869816
REFERENCE   2  (bases 1 to 907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Nov 21. doi: 10.1038/nmeth.4074."
COMMENT     Sequence Label: pTriEx-RhoA-Q63L_mScarlet-i_SGFP2
FEATURES             Location/Qualifiers
     source          1..907
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             600..632
                     /codon_start=1
                     /product="HSV (herpes simplex virus) epitope tag"
                     /label=HSV tag
                     /translation="QPELAPEDPED"
     CDS             639..662
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     primer_bind     complement(752..771)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    817..872
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(871..890)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
ORIGIN
        1 aactggtgat tgttggtgat ggagcctgtg gaaagacatg cttgctcata gtcttcagca
       61 aggaccagtt cccagaggtg tatgtgccca cagtgtttga gaactatgtg gcagatatcg
      121 aggtggatgg aaagcaggta gagttggctt tgtgggacac agctgggctg gaagattatg
      181 atcgcctgag gcccctctcc tacccagata ccgatgttat actgatgtgt ttttccatcg
      241 acagccctga tagtttagaa aacatcccag aaaagtggac cccagaagtc aagcatttct
      301 gtcccaacgt gcccatcatc ctggttggga ataagaagga tcttcggaat gatgagcaca
      361 caaggcggga gcttgccaag atgaagcagg agccggtgaa acctgaagaa ggcagagata
      421 tggcaaacag gattggcgct tttgggtaca tggagtgttc agcaaagacc aaagatggag
      481 tgagagaggt ttttgaaatg gctacgagag ctgctctgca agctagacgt gggaagaaaa
      541 aatctgggtg ccttgtcttg tgaaagcttg cggccgcaca gctgtataca cgtgcaagcc
      601 agccagaact cgccccggaa gaccccgagg atctcgagca ccaccatcac catcaccatc
      661 actaagtgat taacctcagg tgcaggctgc ctatcagaag gtggtggctg gtgtggccaa
      721 tgccctggct cacaaatacc actgagatcg atctttttcc ctctgccaaa aattatgggg
      781 acatcatgaa gccccttgag catctgactt ctggctaata aaggaaattt attttcattg
      841 caatagtgtg ttggaatttt ttgtgtctct cactcggaag gacatatggg agggcaaatc
      901 atttaaa
//
