LOCUS       sequence-167931-         991 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Strain: AM5115, Used for protein purification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Kim YI, Vinyard DJ, Ananyev GM, Dismukes GC, Golden SS
  TITLE     Oxidized quinones signal onset of darkness directly to the 
            cyanobacterial circadian oscillator.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Oct 30;109(44):17765-9. doi: 
            10.1073/pnas.1216401109. Epub 2012 Oct 15.
  PUBMED    23071342
REFERENCE   2  (bases 1 to 991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1216401109"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2012-10-30- 30"; volume: "109"; issue: "44"; pages: "17765-9"
COMMENT     Sequence Label: pET28-SUMO-KaiA
FEATURES             Location/Qualifiers
     source          1..991
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(22..69)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     65..83
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(136..153)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ggatatagtt cctcctttca gcaaaaaacc cctcaagacc cgtttagagg ccccaagggg
       61 ttatgctagt tattgctcag cggtggcagc agccaactca gcttcctttc gggctttgtt
      121 agcagccgga tctcagtggt ggtggtggtg gtgctcgagt gcggccgcaa gctttcaggt
      181 ttctcgtggg atagaccgtc gatacatctc acaaagatgt gcaattacat caattaaagt
      241 cagccgataa tccagcaaaa tgtcctctga acgtccctct acgcggagct tcttggcaaa
      301 ctcgtccatc agctccatgt gaatttctac cactttggtg actggaacat cggcaaagaa
      361 agccatgttg acgaagttgt caatgctctg gttgaggttg ctattcggcg aaaaatagct
      421 caaaacgatt tcacgatagc tagtctgcat cgcttggtgc agcttttggc tttcgtaggc
      481 gggtaggttg cgcagaaagc gatcgggatc acgcttgtag tagaccccga gatagcctag
      541 gcgctcttgt agtcgctgag cgaggtcccg ctgctggctc gatagctcgg gatcgtggtt
      601 ggcccccatc agcatgatgt ggtcggccat ggtctcgacc ggggctaagc gcagaaattc
      661 agccagtgca gcatcaactt ggtagggcaa ttgctcgagc tgatggatac cgaggtgcag
      721 ttcagcgctg tgatagagct gttctttggc tggttcatcg ggatcctcac tgtcgcgatc
      781 gcctacgaca atcgctggta ccaccactcc ctcaaagcag agctgctgaa caactgccct
      841 gaagctggga ttggctgcca ctaaaatcag gcagtcgatt tggtcacgat gggtttgggc
      901 atactccaag agcatttcgc cagactcaca gacttggagt tgatagcgat cggccgacag
      961 cgcccgctgg caatcctgca aaattgccgt c
//
