LOCUS       sequence-167937-         907 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of GFP-SUMO1 in Drosophila cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Mauri F, McNamee LM, Lunardi A, Chiacchiera F, Del Sal G, Brodsky 
            MH, Collavin L
  TITLE     Modification of Drosophila p53 by SUMO modulates its transactivation
            and pro-apoptotic functions.
  JOURNAL   J Biol Chem. 2008 Jul 25. 283(30):20848-56.
  PUBMED    18492669
REFERENCE   2  (bases 1 to 907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2008 Jul 25. 283(30):20848-56."
COMMENT     Sequence Label: pAc-GFP-dSUMO
FEATURES             Location/Qualifiers
     source          1..907
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             335..376
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             386..403
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(426..443)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    476..610
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(500..519)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     554..573
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 tcggcatgga cgagctgtac aagggcggtg gaagatcttc tgacgaaaag aagggaggtg
       61 agaccgagca catcaacctg aaggtcctcg gccaggacaa cgccgtcgtc cagttcaaga
      121 tcaagaagca cacacccttg aggaagctga tgaacgccta ctgcgaccgt gccggactct
      181 ccatgcaggt ggtgcgcttc cgtttcgacg gacagcccat caacgagaac gacactccga
      241 cctcgctgga gatggaggag ggcgacacca tcgaggttta ccagcagcag actggtggcg
      301 ctccataact cgagcctcta gagggccctt cgaaggtaag cctatcccta accctctcct
      361 cggtctcgat tctacgcgta ccggtcatca tcaccatcac cattgagttt aaacccgctg
      421 atcagcctcg actgtgcctt ctaaggcctg agctcgctga tcagcctcga tcgaggatcc
      481 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
      541 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
      601 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg
      661 ggaggttttt taaagcaagt aaaacctcta caaatgtggt atggctgatt atgatcagtc
      721 gaccgatgcc cttgagagcc ttcaacccag tcagctcctt ccggtgggcg cggggcatga
      781 ctatcgtcgc cgcacttatg actgtcttct ttatcatgca actcgtagga caggtgccgg
      841 cagcgctctg ggtcattttc ggcgaggacc gctttcgctg gagcgcgacg atgatcggcc
      901 tgtcgct
//
