LOCUS       sequence-167938-         813 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of GFP-SUMO1 in Drosophila cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Mauri F, McNamee LM, Lunardi A, Chiacchiera F, Del Sal G, Brodsky 
            MH, Collavin L
  TITLE     Modification of Drosophila p53 by SUMO modulates its transactivation
            and pro-apoptotic functions.
  JOURNAL   J Biol Chem. 2008 Jul 25. 283(30):20848-56.
  PUBMED    18492669
REFERENCE   2  (bases 1 to 813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2008 Jul 25. 283(30):20848-56."
COMMENT     Sequence Label: pAc-GFP-dSUMO
FEATURES             Location/Qualifiers
     source          1..813
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     33..50
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(73..90)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(100..141)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(495..516)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ctcgatcgag gctgatcagc gagctcaggc cttagaaggc acagtcgagg ctgatcagcg
       61 ggtttaaact caatggtgat ggtgatgatg accggtacgc gtagaatcga gaccgaggag
      121 agggttaggg ataggcttac cttcgaaggg ccctctagag gctcgagtta tggagcgcca
      181 ccagtctgct gctggtaaac ctcgatggtg tcgccctcct ccatctccag cgaggtcgga
      241 gtgtcgttct cgttgatggg ctgtccgtcg aaacggaagc gcaccacctg catggagagt
      301 ccggcacggt cgcagtaggc gttcatcagc ttcctcaagg gtgtgtgctt cttgatcttg
      361 aactggacga cggcgttgtc ctggccgagg accttcaggt tgatgtgctc ggtctcacct
      421 cccttctttt cgtcagaaga tcttccaccg cccttgtaca gctcgtccat gccgagagtg
      481 atcccggcgg cggtcacgaa ctccagcagg accatgtgat cgcgcttctc gttggggtct
      541 ttgctcaggg cggactgggt gctcaggtag tggttgtcgg gcagcagcac ggggccgtcg
      601 ccgatggggg tgttctgctg gtagtggtcg gcgagctgca cgctgccgtc ctcgatgttg
      661 tggcggatct tgaagttcac cttgatgccg ttcttctgct tgtcggccat gatatagacg
      721 ttgtggctgt tgtagttgta ctccagcttg tgccccagga tgttgccgtc ctccttgaag
      781 tcgatgccct tcagctcgat gcggttcacc agg
//
