LOCUS       sequence-167941-        1007 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Strain: AM5117, Used for protein purification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Kim YI, Vinyard DJ, Ananyev GM, Dismukes GC, Golden SS
  TITLE     Oxidized quinones signal onset of darkness directly to the 
            cyanobacterial circadian oscillator.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Oct 30;109(44):17765-9. doi: 
            10.1073/pnas.1216401109. Epub 2012 Oct 15.
  PUBMED    23071342
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1216401109"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2012-10-30- 30"; volume: "109"; issue: "44"; pages: "17765-9"
COMMENT     Sequence Label: pET41-GST-KaiC
FEATURES             Location/Qualifiers
     source          1..1007
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             39..56
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             66..83
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             99..143
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             174..188
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     CDS             201..224
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 atcctccaaa atcggatggt tcaactagtg gttctggtca tcaccatcac catcactccg
       61 cgggtctggt gccacgcggt agtactgcaa ttggtatgaa agaaaccgct gctgctaaat
      121 tcgaacgcca gcacatggac agcccagatc tgggtaccgg tggtggctcc ggtgatgacg
      181 acgacaagag tcccatggct ctggaagttc tgttccaggg gcccctggga tccatgactt
      241 ccgctgagat gactagccct aataataatt ctgagcacca agccatcgct aagatgcgca
      301 cgatgattga aggctttgat gatattagtc atggcggtct tccaatcggg cgatcgaccc
      361 tcgttagtgg tacttcagga accggcaaga cccttttttc tattcaattt ctctataacg
      421 gtattatcga gtttgatgag cctggggttt tcgttacttt cgaagaaacc ccgcaagata
      481 tcattaaaaa cgcccgtagt tttggctggg atttagccaa gctggtcgat gagggcaaac
      541 tatttattct tgatgcttca cccgatccag aaggtcaaga ggttgttggc ggcttcgatc
      601 tctctgctct gattgagcgg attaattatg caattcaaaa gtatcgagcg cggcgggttt
      661 caattgactc ggtcacgtcc gttttccagc aatatgatgc ctcttctgtg gttcgccgcg
      721 aactctttcg gttggtagct cgcctaaaac aaattggggc aactacggtc atgaccaccg
      781 agcgtatcga ggaatatggc ccgatcgctc gttacggtgt tgaggaattt gtctccgata
      841 acgtcgtgat tctccgcaac gttttggaag gggagcgccg tcgccgcacc ctcgaaatcc
      901 tcaagctacg tggcaccagc cacatgaaag gggaatatcc gttcacgatt acggatcatg
      961 gcatcaatat cttcccgctc ggggcaatgc gccttacgca gcgatcg
//
