LOCUS       sequence-167943-         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of GFP-SUMO1 delta C in Drosophila cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Mauri F, McNamee LM, Lunardi A, Chiacchiera F, Del Sal G, Brodsky 
            MH, Collavin L
  TITLE     Modification of Drosophila p53 by SUMO modulates its transactivation
            and pro-apoptotic functions.
  JOURNAL   J Biol Chem. 2008 Jul 25. 283(30):20848-56.
  PUBMED    18492669
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2008 Jul 25. 283(30):20848-56."
COMMENT     Sequence Label: pAc-GFP-dSUMOdeltaC
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             324..365
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             375..392
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(415..432)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    465..599
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(489..508)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     543..562
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 ctcggcatgg acgagctgta caagggcggt ggaagatctt ctgacgaaaa gaagggaggt
       61 gagaccgagc acatcaacct gaaggtcctc ggccaggaca acgccgtcgt ccagttcaag
      121 atcaagaagc acacaccctt gaggaagctg atgaacgcct actgcgaccg tgccggactc
      181 tccatgcagg tggtgcgctt ccgtttcgac ggacagccca tcaacgagaa cgacactccg
      241 acctcgctgg agatggagga gggcgacacc atcgaggttt accagcagca gacttaactc
      301 gagcctctag agggcccttc gaaggtaagc ctatccctaa ccctctcctc ggtctcgatt
      361 ctacgcgtac cggtcatcat caccatcacc attgagttta aacccgctga tcagcctcga
      421 ctgtgccttc taaggcctga gctcgctgat cagcctcgat cgaggatcca gacatgataa
      481 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
      541 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta
      601 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt
      661 aaagcaagta aaacctctac aaatgtggta tggctgatta tgatcagtcg accgatgccc
      721 ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc
      781 gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg
      841 gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga tgatcggc
//
