LOCUS       sequence-167944-         856 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of GFP-SUMO1 delta C in Drosophila cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Mauri F, McNamee LM, Lunardi A, Chiacchiera F, Del Sal G, Brodsky 
            MH, Collavin L
  TITLE     Modification of Drosophila p53 by SUMO modulates its transactivation
            and pro-apoptotic functions.
  JOURNAL   J Biol Chem. 2008 Jul 25. 283(30):20848-56.
  PUBMED    18492669
REFERENCE   2  (bases 1 to 856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2008 Jul 25. 283(30):20848-56."
COMMENT     Sequence Label: pAc-GFP-dSUMOdeltaC
FEATURES             Location/Qualifiers
     source          1..856
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     33..50
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(73..90)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(100..141)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(483..504)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     832..851
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ctcgatcgag gctgatcagc gagctcaggc cttagaaggc acagtcgagg ctgatcagcg
       61 ggtttaaact caatggtgat ggtgatgatg accggtacgc gtagaatcga gaccgaggag
      121 agggttaggg ataggcttac cttcgaaggg ccctctagag gctcgagtta agtctgctgc
      181 tggtaaacct cgatggtgtc gccctcctcc atctccagcg aggtcggagt gtcgttctcg
      241 ttgatgggct gtccgtcgaa acggaagcgc accacctgca tggagagtcc ggcacggtcg
      301 cagtaggcgt tcatcagctt cctcaagggt gtgtgcttct tgatcttgaa ctggacgacg
      361 gcgttgtcct ggccgaggac cttcaggttg atgtgctcgg tctcacctcc cttcttttcg
      421 tcagaagatc ttccaccgcc cttgtacagc tcgtccatgc cgagagtgat cccggcggcg
      481 gtcacgaact ccagcaggac catgtgatcg cgcttctcgt tggggtcttt gctcagggcg
      541 gactgggtgc tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg
      601 ttctgctggt agtggtcggc gagctgcacg ctgccgtcct cgatgttgtg gcggatcttg
      661 aagttcacct tgatgccgtt cttctgcttg tcggccatga tatagacgtt gtggctgttg
      721 tagttgtact ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc
      781 agctcgatgc ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag
      841 ttgccgtcgt ccttga
//
