LOCUS       sequence-167949-        4038 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing IDH1, part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4038)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4038)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_IDH1_p.P33S
FEATURES             Location/Qualifiers
     source          1..4038
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      11..599
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     500..519
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     753..770
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(929..956)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1048..1134)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1183..1205
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1197..1214
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1198..1214
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1230..1329
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2578..2677)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2694..2713)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2695..2713)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2718..2734)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2718..2734)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3165..3956
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
       61 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
      121 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
      181 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
      241 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
      301 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
      361 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
      421 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
      481 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
      541 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
      601 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
      661 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
      721 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
      781 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
      841 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat acgcgtaccg
      901 ctagccagga agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt
      961 agtttgatgc ctggcagttt atggcgggcg tcctgcccgc caccctccgg gccgttgctt
     1021 cacaacgttc aaatccgctc ccggcggatt tgtcctactc aggagagcgt tcaccgacaa
     1081 acaacagata aaacgaaagg cccagtcttc cgactgagcc tttcgtttta tttgatgcct
     1141 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
     1201 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
     1261 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgacaact ttgtacaaaa
     1321 aagttggcac catgtccaaa aaaatcagtg gcggttctgt ggtagagatg caaggagatg
     1381 aaatgacacg aatcatttgg gaattgatta aagagaaact cattttttcc tacgtggaat
     1441 tggatctaca tagctatgat ttaggcatag agaatcgtga tgccaccaac gaccaagtca
     1501 ccaaggatgc tgcagaagct ataaagaagc ataatgttgg cgtcaaatgt gccactatca
     1561 ctcctgatga gaagagggtt gaggagttca agttgaaaca aatgtggaaa tcaccaaatg
     1621 gcaccatacg aaatattctg ggtggcacgg tcttcagaga agccattatc tgcaaaaata
     1681 tcccccggct tgtgagtgga tgggtaaaac ctatcatcat aggtcgtcat gcttatgggg
     1741 atcaatacag agcaactgat tttgttgttc ctgggcctgg aaaagtagag ataacctaca
     1801 caccaagtga cggaacccaa aaggtgacat acctggtaca taactttgaa gaaggtggtg
     1861 gtgttgccat ggggatgtat aatcaagata agtcaattga agattttgca cacagttcct
     1921 tccaaatggc tctgtctaag ggttggcctt tgtatctgag caccaaaaac actattctga
     1981 agaaatatga tgggcgtttt aaagacatct ttcaggagat atatgacaag cagtacaagt
     2041 cccagtttga agctcaaaag atctggtatg agcataggct catcgacgac atggtggccc
     2101 aagctatgaa atcagaggga ggcttcatct gggcctgtaa aaactatgat ggtgacgtgc
     2161 agtcggactc tgtggcccaa gggtatggct ctctcggcat gatgaccagc gtgctggttt
     2221 gtccagatgg caagacagta gaagcagagg ctgcccacgg gactgtaacc cgtcactacc
     2281 gcatgtacca gaaaggacag gagacgtcca ccaatcccat tgcttccatt tttgcctgga
     2341 ccagagggtt agcccacaga gcaaagcttg ataacaataa agagcttgcc ttctttgcaa
     2401 atgctttgga agaagtctct attgagacaa ttgaggctgg cttcatgacc aaggacttgg
     2461 ctgcttgcat taaaggttta cccaatgtgc aacgttctga ctacttgaat acatttgagt
     2521 tcatggataa acttggagaa aacttgaaga tcaaactagc tcaggccaaa ctttaattgc
     2581 caactttctt gtacaaagtt ggcattataa gaaagcattg cttatcaatt tgttgcaacg
     2641 aacaggtcac tatcagtcaa aataaaatca ttatttgcca tccagctgat atcccctata
     2701 gtgagtcgta ttacatggtc atagctgttt cctggcagct ctggcccgtg tctcaaaatc
     2761 tctgatgtta cattgcacaa gataaaaata tatcatcatg cctcctctag accagccagg
     2821 acagaaatgc ctcgacttcg ctgctaccca aggttgccgg gtgacgcaca ccgtggaaac
     2881 ggatgaaggc acgaacccag tggacataag cctgttcggt tcgtaagctg taatgcaagt
     2941 agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg
     3001 cagtggcggt tttcatggct tgttatgact gtttttttgg ggtacagtct atgcctcggg
     3061 catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg
     3121 atgttacgca gcagggcagt cgccctaaaa caaagttaaa cattatgagg gaagcggtga
     3181 tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac
     3241 cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca
     3301 gtgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt
     3361 tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg
     3421 tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg
     3481 aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca
     3541 cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg
     3601 taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc
     3661 taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg
     3721 tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg
     3781 atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg
     3841 aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt
     3901 tggaagaatt tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc aaataaccct
     3961 cgagccaccc atgaccaaaa tcccttaacg tgagttacgc gtcgttccac tgagcgtcag
     4021 accccgtaga aaagatca
//
