LOCUS       sequence-167954-        3978 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing DCAF4L2 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3978)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 3978)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_DCAF4L2_p.L37H
FEATURES             Location/Qualifiers
     source          1..3978
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1378..1477)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1494..1513)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1495..1513)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1518..1534)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1518..1534)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1965..2756
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2849..3437
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3338..3357
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3591..3608
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3767..3794)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3886..3972)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catggagagc aaaagaccgc gactgctcga ggaagcagac aagcagaaaa
      241 agacagtcag agtgggactc aatgcacctt ccatgctacg aaagaaccag ctaggtttcc
      301 acagattcgc caactattgc cgtatagctc gcgagctgcg tgtaagctgc atgcagagga
      361 aaaaggtcca gattcatagc tgggatccct cctctttggc aagcgaccga tttaaccgca
      421 tactggcgaa taccaacact gaccagctct tcacagtgaa ccaagtcgaa gctggaggct
      481 ccaagtacgg catcatcacc atgcgaggcc tgacgacccc tgagctccgg gtatacccgc
      541 acaaaaccct ctacgtccct aatcggaagg tgaattctat gtgctgggcc tcactgaatc
      601 acttggattc ccaccttctg ctgtgcttcg tgggacttgc agatactcca agctgtgccg
      661 tgctgctccc agcgtcgctg ttcataggta gcttcccagg aatgcgtcgg cctggcatgc
      721 tttgcagttt ccagatccct gatgcctggt cctgtgcctg gtccctgagc atccacgcgt
      781 atcactcttt cagtacaggc ttgtctcagc aggtcctgtt gaccaacgtg gtgacgggac
      841 accagcagtc atttgggact agcagtgatg tcttggccca gcagtttgca atcatgactc
      901 ctttgctgtt taatggctgt cgctctgggg agatctttgg cattgatctg cgctgtggaa
      961 atcaaggcag cgggtggaag gccatttgcc tgtcccatga ttcagcagtg acttctctgc
     1021 aaatcctcca agatggccaa ttcctggtgt catcagacat gactggaact atcaagctgt
     1081 gggacttgag ggccactaaa tgtgtaacac agtacgaagg tcatgtgaat aactccgcct
     1141 acctacccgt gcatgtgaac gaagaagaag gagtcgtggc ggccgtgggc caggactgct
     1201 acacgagaat ctggagcctc cgtcatggcc acctgctcac aaccataccc tccccatacc
     1261 ccgcctcgga gaacgacatt cccagtgtgg ccttctcttc tcgcctcggg ggcttccgag
     1321 gagcaccagg gctgctcatg gctgtccggg aggaccttta ttgtttctcc tacggtttgc
     1381 caactttctt gtacaaagtt ggcattataa gaaagcattg cttatcaatt tgttgcaacg
     1441 aacaggtcac tatcagtcaa aataaaatca ttatttgcca tccagctgat atcccctata
     1501 gtgagtcgta ttacatggtc atagctgttt cctggcagct ctggcccgtg tctcaaaatc
     1561 tctgatgtta cattgcacaa gataaaaata tatcatcatg cctcctctag accagccagg
     1621 acagaaatgc ctcgacttcg ctgctaccca aggttgccgg gtgacgcaca ccgtggaaac
     1681 ggatgaaggc acgaacccag tggacataag cctgttcggt tcgtaagctg taatgcaagt
     1741 agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg
     1801 cagtggcggt tttcatggct tgttatgact gtttttttgg ggtacagtct atgcctcggg
     1861 catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg
     1921 atgttacgca gcagggcagt cgccctaaaa caaagttaaa cattatgagg gaagcggtga
     1981 tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac
     2041 cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca
     2101 gtgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt
     2161 tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg
     2221 tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg
     2281 aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca
     2341 cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg
     2401 taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc
     2461 taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg
     2521 tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg
     2581 atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg
     2641 aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt
     2701 tggaagaatt tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc aaataaccct
     2761 cgagccaccc atgaccaaaa tcccttaacg tgagttacgc gtcgttccac tgagcgtcag
     2821 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     2881 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     2941 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     3001 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     3061 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     3121 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     3181 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     3241 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     3301 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     3361 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     3421 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     3481 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     3541 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     3601 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     3661 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     3721 caattaatac gcgtaccgct agccaggaag agtttgtaga aacgcaaaaa ggccatccgt
     3781 caggatggcc ttctgcttag tttgatgcct ggcagtttat ggcgggcgtc ctgcccgcca
     3841 ccctccgggc cgttgcttca caacgttcaa atccgctccc ggcggatttg tcctactcag
     3901 gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtcttccg actgagcctt
     3961 tcgttttatt tgatgcct
//
