LOCUS       sequence-167956-         818 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of Drosophila p53 in Drosophila cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Mauri F, McNamee LM, Lunardi A, Chiacchiera F, Del Sal G, Brodsky 
            MH, Collavin L
  TITLE     Modification of Drosophila p53 by SUMO modulates its transactivation
            and pro-apoptotic functions.
  JOURNAL   J Biol Chem. 2008 Jul 25. 283(30):20848-56.
  PUBMED    18492669
REFERENCE   2  (bases 1 to 818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2008 Jul 25. 283(30):20848-56."
COMMENT     Sequence Label: pAc-HT-Dmp53
FEATURES             Location/Qualifiers
     source          1..818
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     33..50
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(73..90)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(100..141)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 ctcgatcgag gctgatcagc gagctcaggc cttagaaggc acagtcgagg ctgatcagcg
       61 ggtttaaact caatggtgat ggtgatgatg accggtacgc gtagaatcga gaccgaggag
      121 agggttaggg ataggcttac cttcgaaggg ccctctagag gcgcgcctta tcatggcagc
      181 tcgtaggcac gtttcttaag gctcagcaat ttgttggcat ggcgacgtag attctcttgg
      241 ttgggattgc gcaggacttc agccgccgcc tccttaatca tgccctcgat gctctgcagc
      301 agccattcct tattggggca cgtaatagcc agacggtaat cgccatccgg tgtccgcgac
      361 acgttccact ctgcggcgga gtcgtcgcag tctcggctat cattgctctc cgtgtcctcc
      421 gtctttatag caatgcaccg acgcaccttg gacggctcat cttcttcggc ggcttccggc
      481 acggacttgc gcttcttgct attgagctgg cgttcgtctt ggatgcgatc ccgcttgggg
      541 cacgtacata ttttaacatg tataacatgc tgtcccacga tatcgccgca tgctttctcc
      601 aggcagaaga ctaaggaagt ttcttttcgc ccgatacacg agttttggca gacgaacttg
      661 aaggccaggg tctggcgcgt gagcccactg cgggttacag accggctcat gttcaggggg
      721 actacaacgg aaaaacgctc ggaaattccc ttgccctgag catttccaca atatacactg
      781 ttgggattct cgctgcgcag caagctctcg cgcatttt
//
