LOCUS       sequence-167958-        1067 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 568, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1067)
  TITLE     Davidson lab - mApple constructs
REFERENCE   2  (bases 1 to 1067)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mApple-CytERM-N-17
FEATURES             Location/Qualifiers
     source          1..1067
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      225..234
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             231..941
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     644..663
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 cccatgctag tgaaccgtca gatccgctag cgctaccgga ctcagatctg gtaccccttg
       61 cagcttccag ttgtcaggaa gagaaggctc ccatggatcc tgtggtggtg ctggggctct
      121 gtctctcctg tttgcttctc ctttcactct ggaaacagag ctatggggga gggaagcttc
      181 gaattctgca gtcgacggta ccgcgggccc gggatccacc ggtcgccacc atggtgagca
      241 agggcgagga gaataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg
      301 agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
      361 aggcctttca gaccgctaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg
      421 acatcctgtc ccctcagttc atgtacggct ccaaggtcta cattaagcac ccagccgaca
      481 tccccgacta cttcaagctg tccttccccg agggcttcag gtgggagcgc gtgatgaact
      541 tcgaggacgg cggcattatt cacgttaacc aggactcctc cctgcaggac ggcgtgttca
      601 tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga
      661 agaccatggg ctgggaggcc tccgaggagc ggatgtaccc cgaggacggc gccctgaaga
      721 gcgagatcaa gaagaggctg aagctgaagg acggcggcca ctacgccgcc gaggtcaaga
      781 ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacatcgtc gacatcaagt
      841 tggacatcgt gtcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg
      901 gccgccactc caccggcggc atggacgagc tgtacaagta agcggccgcg actctagatc
      961 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc
     1021 cccctgaacc tgaaacataa aatgatgcat gtgtgtactg ttattgc
//
