LOCUS       sequence-167965-        4605 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing MTM1, part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4605)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4605)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_MTM1_p.P226S
FEATURES             Location/Qualifiers
     source          1..4605
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     303..322
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     556..573
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(732..759)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(851..937)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     986..1008
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1000..1017
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1001..1017
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1033..1132
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2948..3047)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3064..3083)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3065..3083)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3088..3104)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3088..3104)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3535..4326
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(4419..4605,1..402)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
       61 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
      121 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
      181 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
      241 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
      301 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
      361 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
      421 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
      481 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
      541 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
      601 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
      661 tggaaagcgg gcagtgagcg caacgcaatt aatacgcgta ccgctagcca ggaagagttt
      721 gtagaaacgc aaaaaggcca tccgtcagga tggccttctg cttagtttga tgcctggcag
      781 tttatggcgg gcgtcctgcc cgccaccctc cgggccgttg cttcacaacg ttcaaatccg
      841 ctcccggcgg atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa
      901 aggcccagtc ttccgactga gcctttcgtt ttatttgatg cctggcagtt ccctactctc
      961 gcgttaacgc tagcatggat gttttcccag tcacgacgtt gtaaaacgac ggccagtctt
     1021 aagctcgggc cccaaataat gattttattt tgactgatag tgacctgttc gttgcaacaa
     1081 attgatgagc aatgcttttt tataatgcca actttgtaca aaaaagttgg caccatggct
     1141 tctgcatcaa cttctaaata taattcacac tccttggaga atgagtctat taagaggacg
     1201 tctcgagatg gagtcaatcg agatctcact gaggctgttc ctcgacttcc aggagaaaca
     1261 ctaatcactg acaaagaagt tatttacata tgtcctttca atggccccat taagggaaga
     1321 gtttacatca caaattatcg tctttattta agaagtttgg aaacggattc ttctctaata
     1381 cttgatgttc ctctgggtgt gatctcgaga attgaaaaaa tgggaggcgc gacaagtaga
     1441 ggagaaaatt cctatggtct agatattact tgtaaagaca tgagaaacct gaggttcgct
     1501 ttgaaacagg aaggccacag cagaagagat atgtttgaga tcctcacgag atacgcgttt
     1561 cccctggctc acagtctgcc attatttgca tttttaaatg aagaaaagtt taacgtggat
     1621 ggatggacag tttacaatcc agtggaagaa tacaggaggc agggcttgcc caatcaccat
     1681 tggagaataa cttttattaa taagtgctat gagctctgtg acacttaccc tgctcttttg
     1741 gtggttccgt atcgtgcctc agatgatgac ctccggagag ttgcaacttt taggtcccga
     1801 aatcgaattt cagtgctgtc atggattcat ccagaaaata agacggtcat tgtgcgttgc
     1861 agtcagcctc ttgtcggtat gagtgggaaa cgaaataaag atgatgagaa atatctcgat
     1921 gttatcaggg agactaataa acaaatttct aaactcacca tttatgatgc aagacccagc
     1981 gtaaatgcag tggccaacaa ggcaacagga ggaggatatg aaagtgatga tgcatatcat
     2041 aacgccgaac ttttcttctt agacattcat aatattcatg ttatgcggga atctttaaaa
     2101 aaagtgaagg acattgttta tcctaatgta gaagaatctc attggttgtc cagtttggag
     2161 tctactcatt ggttagaaca tatcaagctc gttttgacag gagccattca agtagcagac
     2221 aaagtttctt cagggaagag ttcagtgctt gtgcattgca gtgacggatg ggacaggact
     2281 gctcagctga catccttggc catgctgatg ttggatagct tctataggag cattgaaggg
     2341 ttcgaaatac tggtacaaaa aaaatggata agttttggac ataaatttgc atctcgaata
     2401 ggtcatggtg ataaaaacca caccgatgct gaccgttctc ctatttttct ccagtttatt
     2461 gattgtgtgt ggcaaatgtc aaaacagttc cctacagctt ttgaattcaa tgaacaattt
     2521 ttgattataa ttttggatca tctgtatagt tgccgatttg gtactttctt attcaactgt
     2581 gaatctgctc gagaaagaca gaaggttaca gaaaggactg tttctttatg gtcactgata
     2641 aacagtaata aagaaaaatt caaaaacccc ttctatacta aagaaatcaa tcgagtttta
     2701 tatccagttg ccagtatgcg tcacttggaa ctctgggtga attactacat tagatggaac
     2761 cccaggatca agcaacaaca gccgaatcca gtggagcagc gttacatgga gctcttagcc
     2821 ttacgcgacg aatacataaa gcggcttgag gaactgcagc tcgccaactc tgccaagctt
     2881 tctgatcccc caacttcacc ttccagtcct tcgcaaatga tgccccatgt gcaaactcac
     2941 ttctagttgc caactttctt gtacaaagtt ggcattataa gaaagcattg cttatcaatt
     3001 tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttgcca tccagctgat
     3061 atcccctata gtgagtcgta ttacatggtc atagctgttt cctggcagct ctggcccgtg
     3121 tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg cctcctctag
     3181 accagccagg acagaaatgc ctcgacttcg ctgctaccca aggttgccgg gtgacgcaca
     3241 ccgtggaaac ggatgaaggc acgaacccag tggacataag cctgttcggt tcgtaagctg
     3301 taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt
     3361 ggtaacggcg cagtggcggt tttcatggct tgttatgact gtttttttgg ggtacagtct
     3421 atgcctcggg catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg
     3481 agcagcaacg atgttacgca gcagggcagt cgccctaaaa caaagttaaa cattatgagg
     3541 gaagcggtga tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc
     3601 catctcgaac cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg
     3661 aagccacaca gtgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg
     3721 cggcgagctt tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt
     3781 ctccgcgctg tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca
     3841 gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc
     3901 gagccagcca cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc
     3961 gttgccttgg taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta
     4021 tttgaggcgc taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat
     4081 gagcgaaatg tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc
     4141 gcgccgaagg atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc
     4201 gtcatacttg aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc
     4261 gcagatcagt tggaagaatt tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc
     4321 aaataaccct cgagccaccc atgaccaaaa tcccttaacg tgagttacgc gtcgttccac
     4381 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     4441 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     4501 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     4561 actgttcttc tagtgtagcc gtagttaggc caccacttca agaac
//
