LOCUS       sequence-167974-        1014 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Minimos transposon with Peft-3:GFP:tbb-2 3'UTR and cbr-unc-119 
            selection. GFP was optimized for C. elegans, contains 2x NLS 
            (SV40/egl-13), and smu-2 introns.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Frokjaer-Jensen C, Jain N, Hansen L, Davis MW, Li Y, Zhao D, Rebora 
            K, Millet JR, Liu X, Kim SK, Dupuy D, Jorgensen EM, Fire AZ
  TITLE     An Abundant Class of Non-coding DNA Can Prevent Stochastic Gene 
            Silencing in the C. elegans Germline.
  JOURNAL   Cell. 2016 Jul 14;166(2):343-57. doi: 10.1016/j.cell.2016.05.072. 
            Epub 2016 Jun 30.
  PUBMED    27374334
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.05.072"; journalName: "Cell"; date: 
            "2016-07-14- 14"; volume: "166"; issue: "2"; pages: "343-57"
COMMENT     Sequence Label: pCFJ1320
FEATURES             Location/Qualifiers
     source          1..1014
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             39..110
                     /codon_start=1
                     /product="nuclear localization signal from the C. elegans 
                     EGL-13 transcription factor (Lyssenko et al., 2007)"
                     /label=EGL-13 NLS
                     /translation="SRRRKANPTKLSENAKKLAKEVEN"
     protein_bind    complement(112..136)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      141..464
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    complement(468..488)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(496..513)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(496..512)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ccgccggaat cacccacgga atggacgagc tctacaagtc ccgtcgtcgt aaggccaacc
       61 caaccaagct ctccgagaac gccaagaagc tcgccaagga ggtcgagaac aacccagctt
      121 tcttgtacaa agtgggataa atgcaaaatc ctttcaagca ttcccttctt ctctatcact
      181 cttctttctt tttgtcaaaa aattctctcg ctaatttatt tgctttttta atgttattat
      241 tttatgactt tttatagtca ctgaaaagtt tgcatctgag tgaagtgaat gctatcaaaa
      301 tgtgattctg tctgatgtac tttcacaatc tctcttcaat tccattttga agtgctttaa
      361 acccgaaagg ttgagaaaaa tgcgagcgct caaatatttg tattgtgttc gttgagtgac
      421 ccaacaaaaa gaggaaactt tattgtgccg ccaagaaaaa agtctcacaa ctttattata
      481 catagttgat aattcactgg ccgtcgtttt acactcgaga cgtacggtgc gcgcgatgca
      541 ttcgaagatc tgcccactag tgagtcgtat tataagtgca agtaagatca gtgtttgttt
      601 cgatatcagt ctgtttcgta acggtcttct gtataactac aaaaaagaat aaaaaaccgt
      661 atcacaactg agaaaaaaga caaaacttat tgaacagtgt tcctttcaaa aaattatttt
      721 gtaaaaaatc gagtttttct ccaaaataac gcacgcaatc aaaaacaaga aaactaacgt
      781 gttctcctgg gcgtggaact ccttatcaag attaccatta ttgtctgtca gaattgatat
      841 ttcaattatt tcatatctag acaaacccgg gtgtcttgca tttattagta ctgtgagttt
      901 acatacaaac gaggcttact cagataacat agagtcacct ctaattagca aaaacccatt
      961 tctaattagc tatttcgacc agttctaaag tcaccgtaat tagaaatggt tttt
//
