LOCUS       sequence-167975-        1034 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Minimos transposon with Peft-3:tdTomato:tbb-2 3'UTR and cbr-unc-119 
            selection. tdTomato was optimized for C. elegans, contains 2x NLS 
            (SV40/egl-13), and synthetic introns.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Frokjaer-Jensen C, Jain N, Hansen L, Davis MW, Li Y, Zhao D, Rebora 
            K, Millet JR, Liu X, Kim SK, Dupuy D, Jorgensen EM, Fire AZ
  TITLE     An Abundant Class of Non-coding DNA Can Prevent Stochastic Gene 
            Silencing in the C. elegans Germline.
  JOURNAL   Cell. 2016 Jul 14;166(2):343-57. doi: 10.1016/j.cell.2016.05.072. 
            Epub 2016 Jun 30.
  PUBMED    27374334
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.05.072"; journalName: "Cell"; date: 
            "2016-07-14- 14"; volume: "166"; issue: "2"; pages: "343-57"
COMMENT     Sequence Label: pCFJ1955
FEATURES             Location/Qualifiers
     source          1..1034
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    247..267
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        268..867
                     /gene="eft-3"
                     /label=eft-3 promoter
                     /note="promoter for the C. elegans elongation factor 
                     1-alpha gene (Mitrovich and Anderson, 2000)"
     protein_bind    893..917
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cccccttgtt tctatatttt ttcgctgtcc tgtcacactc gctaaaaaca ctaatcacac
       61 ggaaatctgt tttcaataaa aactccaact ttaactcatt ttcaatttca actgaaagat
      121 tttttcatta gagaatgtct agaactaggc ccgggctacg taatacgact cacttaaggc
      181 cttgactaga gggtaccaga gctcacctag gcaggaacag ctatgaccat gattacgcca
      241 agctatcaac tttgtataga aaagttggca cctttggtct tttattgtca acttccattg
      301 gttcttccat tgtttctgtt aaattaatga atttttcata aaataaagac attatacaat
      361 ataaaaatga agaatttatt gaaaataaac tgccagagag aaaaagtatg caacactccc
      421 gccgagagtg tttgaaatgg tgtacggtac attttcgtgc taggagttag atgtgcaggc
      481 agcaacgaga gggggagaga tttttttggg ccttgtgaaa ttaacgtgag ttttctggtc
      541 atctgactaa tcatgttggt tttttgttgg tttattttgt ttttatcttt gtttttatcc
      601 agattaggaa atttaaattt tatgaattta taatgaggtc aaacattcag tcccagcgtt
      661 tttcctgttc tcactgttta gtcgaatttt tattttaggc tttcaacaaa tgttctaact
      721 gtcttatttg tgacctcact ttttatattt ttttaatttt taaaaatatt agaagtttct
      781 aggataattt tttcgacttt tattctctct accgtccgca ctcttcttac ttttaaatta
      841 aattgttttt ttttcagttg ggaaacactt tgctcactcc gtagcagcca tggcaagttt
      901 gtacaaaaaa gcaggctcgc caaaggtaag tttatctaaa aagtttttca ttcaaaatgt
      961 gtaaaaattc atttaaaata acccaaaaat cattaatcct cgatattttc agaagaagcg
     1021 taaggtcgtt tcca
//
