LOCUS       sequence-167977-        1006 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Minimos transposon with Peft-3:tdTomato:tbb-2 3'UTR and cbr-unc-119 
            selection. tdTomato was optimized for C. elegans, contains 2x NLS 
            (SV40/egl-13), and synthetic introns.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Frokjaer-Jensen C, Jain N, Hansen L, Davis MW, Li Y, Zhao D, Rebora 
            K, Millet JR, Liu X, Kim SK, Dupuy D, Jorgensen EM, Fire AZ
  TITLE     An Abundant Class of Non-coding DNA Can Prevent Stochastic Gene 
            Silencing in the C. elegans Germline.
  JOURNAL   Cell. 2016 Jul 14;166(2):343-57. doi: 10.1016/j.cell.2016.05.072. 
            Epub 2016 Jun 30.
  PUBMED    27374334
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.05.072"; journalName: "Cell"; date: 
            "2016-07-14- 14"; volume: "166"; issue: "2"; pages: "343-57"
COMMENT     Sequence Label: pCFJ1955
FEATURES             Location/Qualifiers
     source          1..1006
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(15..338)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    343..367
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(369..440)
                     /codon_start=1
                     /product="nuclear localization signal from the C. elegans 
                     EGL-13 transcription factor (Lyssenko et al., 2007)"
                     /label=EGL-13 NLS
                     /translation="SRRRKANPTKLSENAKKLAKEVEN"
ORIGIN
        1 taataaagtt gtgagacttt tttcttggcg gcacaataaa gtttcctctt tttgttgggt
       61 cactcaacga acacaataca aatatttgag cgctcgcatt tttctcaacc tttcgggttt
      121 aaagcacttc aaaatggaat tgaagagaga ttgtgaaagt acatcagaca gaatcacatt
      181 ttgatagcat tcacttcact cagatgcaaa cttttcagtg actataaaaa gtcataaaat
      241 aataacatta aaaaagcaaa taaattagcg agagaatttt ttgacaaaaa gaaagaagag
      301 tgatagagaa gaagggaatg cttgaaagga ttttgcattt atcccacttt gtacaagaaa
      361 gctgggttgt tctcgacctc cttggcgagc ttcttggcgt tctcggagag cttggttggg
      421 ttggccttac gacgacggga cttgtagagc tcgtccattc cgaggaagag gtggtgacgt
      481 ccctcggaac gctcgtattg ctcgacgatg gtgtagtcct cgttgtggga ggtgatgtcg
      541 agcttggtgt cgacgtagta gtatcctggg agttggactg gcttcttggc catgtagatg
      601 gtcttgaact cgacgaggta gtgtcctccg tcctgaaaat ctatgagacc tagttagtta
      661 gtaaaggtct ctagaaaact taccttgagc ttgagggctt ggtggatctc tcccttgagg
      721 actccgtcac gtgggtagag gcgctcggtg gaggcctccc atcccatggt cttcttttgc
      781 atgactggtc cgtctggtgg gaagttggtt ccacgcatct gaaaaattct gagacctagt
      841 tagttagtaa aggtctctta tgaacttacc ttgactttgt atatgagggt tccgtcttgg
      901 agggaggagt cttgggtgac ggtgacgagt cctccgtcct cgaagttcat gacacgctcc
      961 cacttgaatc cctctgggaa ggagagcttc tgaaaattac tgagac
//
