LOCUS       sequence-167979-        1048 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Minimos transposon with Peft-3:GFP:tbb-2 3'UTR and cbr-unc-119 
            selection. GFP was optimized for C. elegans, contains 2x NLS 
            (SV40/egl-13), and synthetic introns.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Frokjaer-Jensen C, Jain N, Hansen L, Davis MW, Li Y, Zhao D, Rebora 
            K, Millet JR, Liu X, Kim SK, Dupuy D, Jorgensen EM, Fire AZ
  TITLE     An Abundant Class of Non-coding DNA Can Prevent Stochastic Gene 
            Silencing in the C. elegans Germline.
  JOURNAL   Cell. 2016 Jul 14;166(2):343-57. doi: 10.1016/j.cell.2016.05.072. 
            Epub 2016 Jun 30.
  PUBMED    27374334
REFERENCE   2  (bases 1 to 1048)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.05.072"; journalName: "Cell"; date: 
            "2016-07-14- 14"; volume: "166"; issue: "2"; pages: "343-57"
COMMENT     Sequence Label: pCFJ1137
FEATURES             Location/Qualifiers
     source          1..1048
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(153..173)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(176..200)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(226..825)
                     /gene="eft-3"
                     /label=eft-3 promoter
                     /note="promoter for the C. elegans elongation factor 
                     1-alpha gene (Mitrovich and Anderson, 2000)"
     protein_bind    complement(826..846)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 ccctctccct ctccggagac ggagaacttg tgtccgttga cgtctccgtc gagctcgacg
       61 aggattggga cgactccggt gaagagctcc tctccctgaa aatctatgag acctagttag
      121 ttagtaaagg tctctagaaa acttaccttg gacaccttac gcttcttctt tggcgagcct
      181 gcttttttgt acaaacttgc catggctgct acggagtgag caaagtgttt cccaactgaa
      241 aaaaaaacaa tttaatttaa aagtaagaag agtgcggacg gtagagagaa taaaagtcga
      301 aaaaattatc ctagaaactt ctaatatttt taaaaattaa aaaaatataa aaagtgaggt
      361 cacaaataag acagttagaa catttgttga aagcctaaaa taaaaattcg actaaacagt
      421 gagaacagga aaaacgctgg gactgaatgt ttgacctcat tataaattca taaaatttaa
      481 atttcctaat ctggataaaa acaaagataa aaacaaaata aaccaacaaa aaaccaacat
      541 gattagtcag atgaccagaa aactcacgtt aatttcacaa ggcccaaaaa aatctctccc
      601 cctctcgttg ctgcctgcac atctaactcc tagcacgaaa atgtaccgta caccatttca
      661 aacactctcg gcgggagtgt tgcatacttt ttctctctgg cagtttattt tcaataaatt
      721 cttcattttt atattgtata atgtctttat tttatgaaaa attcattaat ttaacagaaa
      781 caatggaaga accaatggaa gttgacaata aaagaccaaa ggtgccaact tttctataca
      841 aagttgatag cttggcgtaa tcatggtcat agctgttcct gcctaggtga gctctggtac
      901 cctctagtca aggccttaag tgagtcgtat tacgtagccc gggcctagtt ctagacattc
      961 tctaatgaaa aaatctttca gttgaaattg aaaatgagtt aaagttggag tttttattga
     1021 aaacagattt ccgtgtgatt agtgtttt
//
