LOCUS       sequence-167981-        1053 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Minimos transposon with Peft-3:GFP:tbb-2 3'UTR and cbr-unc-119 
            selection. GFP was optimized for C. elegans, contains 2x NLS 
            (SV40/egl-13), and synthetic introns.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Frokjaer-Jensen C, Jain N, Hansen L, Davis MW, Li Y, Zhao D, Rebora 
            K, Millet JR, Liu X, Kim SK, Dupuy D, Jorgensen EM, Fire AZ
  TITLE     An Abundant Class of Non-coding DNA Can Prevent Stochastic Gene 
            Silencing in the C. elegans Germline.
  JOURNAL   Cell. 2016 Jul 14;166(2):343-57. doi: 10.1016/j.cell.2016.05.072. 
            Epub 2016 Jun 30.
  PUBMED    27374334
REFERENCE   2  (bases 1 to 1053)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.05.072"; journalName: "Cell"; date: 
            "2016-07-14- 14"; volume: "166"; issue: "2"; pages: "343-57"
COMMENT     Sequence Label: pCFJ1137
FEATURES             Location/Qualifiers
     source          1..1053
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             478..549
                     /codon_start=1
                     /product="nuclear localization signal from the C. elegans 
                     EGL-13 transcription factor (Lyssenko et al., 2007)"
                     /label=EGL-13 NLS
                     /translation="SRRRKANPTKLSENAKKLAKEVEN"
     protein_bind    complement(551..575)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      580..903
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    complement(907..927)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(935..952)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(935..951)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ttcgagggag acaccctcgt caaccgtatc gagctcaagg gaatcgactt caaggaggac
       61 ggaaacatcc tcggacacaa gctcgagtac aactacaact cccacaacgt ctacatcatg
      121 gccgacaagc aaaagaacgg aatcaaggtc aacttcaagg taagttcttt agagaccttt
      181 actaactaac taggtctcag taattttcag atccgtcaca acatcgagga cggatccgtc
      241 caactcgccg accactacca acaaaacacc ccaatcggag acggaccagt cctcctccca
      301 gacaaccact acctctccac ccaatccgcc ctctccaagg taagttcata agagaccttt
      361 actaactaac taggtctcag aatttttcag gacccaaacg agaagcgtga ccacatggtc
      421 ctcctcgagt tcgtcaccgc cgccggaatc acccacggaa tggacgagct ctacaagtcc
      481 cgtcgtcgta aggccaaccc aaccaagctc tccgagaacg ccaagaagct cgccaaggag
      541 gtcgagaaca acccagcttt cttgtacaaa gtgggataaa tgcaaaatcc tttcaagcat
      601 tcccttcttc tctatcactc ttctttcttt ttgtcaaaaa attctctcgc taatttattt
      661 gcttttttaa tgttattatt ttatgacttt ttatagtcac tgaaaagttt gcatctgagt
      721 gaagtgaatg ctatcaaaat gtgattctgt ctgatgtact ttcacaatct ctcttcaatt
      781 ccattttgaa gtgctttaaa cccgaaaggt tgagaaaaat gcgagcgctc aaatatttgt
      841 attgtgttcg ttgagtgacc caacaaaaag aggaaacttt attgtgccgc caagaaaaaa
      901 gtctcacaac tttattatac atagttgata attcactggc cgtcgtttta cactcgagac
      961 gtacggtgcg cgcgatgcat tcgaagatct gcccactagt gagtcgtatt ataagtgcaa
     1021 gtaagatcag tgtttgtttc gatatcagtc tgt
//
