LOCUS       sequence-167982-        4005 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing PTEN, part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4005)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4005)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_PTEN_p.G129V
FEATURES             Location/Qualifiers
     source          1..4005
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             55..846
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      939..1527
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1428..1447
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1681..1698
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(1857..1884)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1976..2062)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2111..2133
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2125..2142
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2126..2142
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2158..2257
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3473..3572)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3589..3608)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3590..3608)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3613..3629)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3613..3629)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 agcagcaacg atgttacgca gcagggcagt cgccctaaaa caaagttaaa cattatgagg
       61 gaagcggtga tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc
      121 catctcgaac cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg
      181 aagccacaca gtgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg
      241 cggcgagctt tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt
      301 ctccgcgctg tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca
      361 gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc
      421 gagccagcca cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc
      481 gttgccttgg taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta
      541 tttgaggcgc taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat
      601 gagcgaaatg tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc
      661 gcgccgaagg atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc
      721 gtcatacttg aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc
      781 gcagatcagt tggaagaatt tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc
      841 aaataaccct cgagccaccc atgaccaaaa tcccttaacg tgagttacgc gtcgttccac
      901 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
      961 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     1021 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     1081 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     1141 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     1201 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     1261 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     1321 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     1381 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     1441 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     1501 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     1561 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     1621 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     1681 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     1741 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     1801 gagcgcaacg caattaatac gcgtaccgct agccaggaag agtttgtaga aacgcaaaaa
     1861 ggccatccgt caggatggcc ttctgcttag tttgatgcct ggcagtttat ggcgggcgtc
     1921 ctgcccgcca ccctccgggc cgttgcttca caacgttcaa atccgctccc ggcggatttg
     1981 tcctactcag gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtcttccg
     2041 actgagcctt tcgttttatt tgatgcctgg cagttcccta ctctcgcgtt aacgctagca
     2101 tggatgtttt cccagtcacg acgttgtaaa acgacggcca gtcttaagct cgggccccaa
     2161 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga tgagcaatgc
     2221 ttttttataa tgccaacttt gtacaaaaaa gttggcacca tgacagccat catcaaagag
     2281 atcgttagca gaaacaaaag gagatatcaa gaggatggat tcgacttaga cttgacctat
     2341 atttatccaa acattattgc tatgggattt cctgcagaaa gacttgaagg cgtatacagg
     2401 aacaatattg atgatgtagt aaggtttttg gattcaaagc ataaaaacca ttacaagata
     2461 tacaatcttt gtgctgaaag acattatgac accgccaaat ttaattgcag agttgcacaa
     2521 tatccttttg aagaccataa cccaccacag ctagaactta tcaaaccctt ttgtgaagat
     2581 cttgaccaat ggctaagtga agatgacaat catgttgcag caattcactg taaagctgga
     2641 aaggtacgaa ctggtgtaat gatatgtgca tatttattac atcggggcaa atttttaaag
     2701 gcacaagagg ccctagattt ctatggggaa gtaaggacca gagacaaaaa gggagtaact
     2761 attcccagtc agaggcgcta tgtgtattat tatagctacc tgttaaagaa tcatctggat
     2821 tatagaccag tggcactgtt gtttcacaag atgatgtttg aaactattcc aatgttcagt
     2881 ggcggaactt gcaatcctca gtttgtggtc tgccagctaa aggtgaagat atattcctcc
     2941 aattcaggac ccacacgacg ggaagacaag ttcatgtact ttgagttccc tcagccgtta
     3001 cctgtgtgtg gtgatatcaa agtagagttc ttccacaaac agaacaagat gctaaaaaag
     3061 gacaaaatgt ttcacttttg ggtaaataca ttcttcatac caggaccaga ggaaacctca
     3121 gaaaaagtag aaaatggaag tctatgtgat caagaaatcg atagcatttg cagtatagag
     3181 cgtgcagata atgacaagga atatctagta cttactttaa caaaaaatga tcttgacaaa
     3241 gcaaataaag acaaagccaa ccgatacttt tctccaaatt ttaaggtgaa gctgtacttc
     3301 acaaaaacag tagaggagcc gtcaaatcca gaggctagca gttcaacttc tgtaacacca
     3361 gatgttagtg acaatgaacc tgatcattat agatattctg acaccactga ctctgatcca
     3421 gagaatgaac cttttgatga agatcagcat acacaaatta caaaagtctg attgccaact
     3481 ttcttgtaca aagttggcat tataagaaag cattgcttat caatttgttg caacgaacag
     3541 gtcactatca gtcaaaataa aatcattatt tgccatccag ctgatatccc ctatagtgag
     3601 tcgtattaca tggtcatagc tgtttcctgg cagctctggc ccgtgtctca aaatctctga
     3661 tgttacattg cacaagataa aaatatatca tcatgcctcc tctagaccag ccaggacaga
     3721 aatgcctcga cttcgctgct acccaaggtt gccgggtgac gcacaccgtg gaaacggatg
     3781 aaggcacgaa cccagtggac ataagcctgt tcggttcgta agctgtaatg caagtagcgt
     3841 atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg
     3901 gcggttttca tggcttgtta tgactgtttt tttggggtac agtctatgcc tcgggcatcc
     3961 aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatgg
//
