LOCUS       sequence-167986-        1016 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Minimos transposon with Peft-3:GFP:tbb-2 3'UTR and cbr-unc-119 
            selection. GFP was optimized for C. elegans, contains 2x NLS 
            (SV40/egl-13), and smu-1 introns.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Frokjaer-Jensen C, Jain N, Hansen L, Davis MW, Li Y, Zhao D, Rebora 
            K, Millet JR, Liu X, Kim SK, Dupuy D, Jorgensen EM, Fire AZ
  TITLE     An Abundant Class of Non-coding DNA Can Prevent Stochastic Gene 
            Silencing in the C. elegans Germline.
  JOURNAL   Cell. 2016 Jul 14;166(2):343-57. doi: 10.1016/j.cell.2016.05.072. 
            Epub 2016 Jun 30.
  PUBMED    27374334
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.05.072"; journalName: "Cell"; date: 
            "2016-07-14- 14"; volume: "166"; issue: "2"; pages: "343-57"
COMMENT     Sequence Label: pCFJ1415
FEATURES             Location/Qualifiers
     source          1..1016
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(14..337)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    342..366
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(368..439)
                     /codon_start=1
                     /product="nuclear localization signal from the C. elegans 
                     EGL-13 transcription factor (Lyssenko et al., 2007)"
                     /label=EGL-13 NLS
                     /translation="SRRRKANPTKLSENAKKLAKEVEN"
ORIGIN
        1 aataaagttg tgagactttt ttcttggcgg cacaataaag tttcctcttt ttgttgggtc
       61 actcaacgaa cacaatacaa atatttgagc gctcgcattt ttctcaacct ttcgggttta
      121 aagcacttca aaatggaatt gaagagagat tgtgaaagta catcagacag aatcacattt
      181 tgatagcatt cacttcactc agatgcaaac ttttcagtga ctataaaaag tcataaaata
      241 ataacattaa aaaagcaaat aaattagcga gagaattttt tgacaaaaag aaagaagagt
      301 gatagagaag aagggaatgc ttgaaaggat tttgcattta tcccactttg tacaagaaag
      361 ctgggttgtt ctcgacctcc ttggcgagct tcttggcgtt ctcggagagc ttggttgggt
      421 tggccttacg acgacgggac ttgtagagct cgtccattcc gtgggtgatt ccggcggcgg
      481 tgacgaactc gaggaggacc atgtggtcac gcttctcgtt tgggtcctga aaaattcttt
      541 ttttattgat ttttagcatt gaaaacatga atttcagtta aaaatctttt tgaaaaatgg
      601 aaatatactg gaaaacggtc ccgaaaattg gggaaagatt tttagaacac ataatgtgaa
      661 taaaaagctg gaattttaaa tttttcaacc aaaatttaga aaaagtatga aacaatactt
      721 tttcataatt ttatttttta atttacactt tacacaattt aaacatattt ttttaatttt
      781 tagaaacatt ttttgcattg aaaaatgtac cttttcaaac aaatatacat ttttcaatat
      841 tttaattttt tttactttca ttttttgtgt aattaaaagc atcttaaatt tttttttgca
      901 tttttttaaa cctttttttt actgttttgg aaacattctt aggcgctttt tttgttaatt
      961 ttacatttaa aaatgctctt aaatattttt attccatttt tcagttctta aacttt
//
