LOCUS       sequence-168014-        3699 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Can be used for in situ hybridisation or other purposes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3699)
  AUTHORS   Hall TE, Martel N, Lo HP, Xiong Z, Parton RG
  TITLE     A plasmid library of full-length zebrafish rab proteins for in vivo 
            cell biology.
  JOURNAL   Cell Logist. 2017 Mar 1;7(1):e1301151. doi: 
            10.1080/21592799.2017.1301151. eCollection 2017.
  PUBMED    28396820
REFERENCE   2  (bases 1 to 3699)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2017-03-1"; volume: "7(1):e1301151. doi"; pages: " 
            10.1080/21592799.2017.1301151. eCollection 2017"
COMMENT     Sequence Label: Rab11ba cds in pGem-T Easy
FEATURES             Location/Qualifiers
     source          1..3699
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      37..46
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(824..842)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(825..842)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(860..876)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(860..876)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(873..895)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    884..900
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(908..914,915..932,933..938))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    953..974
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1091..1108)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1262..1850)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1342..1361)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2021..2812,2813..2881))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2644..2663
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2882..2986)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3064..3519)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3201..3222)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3413..3432
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3645..3667
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3659..3676
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3660..3676
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(3683..3699,1..3)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        join(3683..3699,1..2)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggcgaattg ggcccgacgt cgcatgctcc cggccgccat ggcggccgcg ggaattcgat
       61 tcagtgcggt taaccaacca tggggacccg tgacgacgaa tacgattatt tatttaaagt
      121 ggttctcatt ggagactctg gggtggggaa gagtaacctg ttgtcccgtt tcactcggaa
      181 cgagtttaac ctggagagca agagcaccat tggtgtggag ttcgcaaccc gcagcatcca
      241 agtggacggg aagaccatta aagctcagat ctgggataca gcaggacagg aacgctacag
      301 agcaataaca tcagcgtact accggggtgc agttggagct cttctggtct acgacatcgc
      361 aaaacatctg acttacgaga atgtggagcg ctggctgaag gagctgcggg atcatgcgga
      421 taacaacatc gtcatcatgc tggtgggcaa caagagcgac ctgaggcacc tgagggccgt
      481 gcccactgat gaggccagag cttttgcaga gaagaacaat ctctccttca ttgaaacatc
      541 ggcgttggac tccacaaatg tggaggaggc atttaaaaac atactaacag aaatctatcg
      601 gattgtgtca cagaaacaga tggtcgacag gcccgggcac gatgagtctc ctggcaataa
      661 cgtagtggac atcagcgtgc cgcccactac tgacgggtta aagggcaaca aatttcagtg
      721 ctgtcagaac ctgtgaccca acaaatcact agtgaattcg cggccgcctg caggtcgacc
      781 atatgggaga gctcccaacg cgttggatgc atagcttgag tattctatag tgtcacctaa
      841 atagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
      901 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
      961 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     1021 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     1081 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     1141 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     1201 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     1261 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     1321 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     1381 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     1441 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     1501 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     1561 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     1621 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     1681 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     1741 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     1801 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     1861 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     1921 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     1981 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     2041 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     2101 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     2161 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     2221 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     2281 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     2341 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     2401 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     2461 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     2521 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     2581 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     2641 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     2701 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     2761 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     2821 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     2881 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     2941 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     3001 aagtgccacc tgatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
     3061 aggaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct
     3121 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg
     3181 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact
     3241 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac
     3301 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga
     3361 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga
     3421 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca
     3481 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc cattcgccat tcaggctgcg
     3541 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
     3601 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
     3661 taaaacgacg gccagtgaat tgtaatacga ctcactata
//
