LOCUS       sequence-168018-        3682 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Can be used for in situ hybridisation or other purposes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3682)
  AUTHORS   Hall TE, Martel N, Lo HP, Xiong Z, Parton RG
  TITLE     A plasmid library of full-length zebrafish rab proteins for in vivo 
            cell biology.
  JOURNAL   Cell Logist. 2017 Mar 1;7(1):e1301151. doi: 
            10.1080/21592799.2017.1301151. eCollection 2017.
  PUBMED    28396820
REFERENCE   2  (bases 1 to 3682)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2017-03-1"; volume: "7(1):e1301151. doi"; pages: " 
            10.1080/21592799.2017.1301151. eCollection 2017"
COMMENT     Sequence Label: Rab14 cds in pGem-T Easy
FEATURES             Location/Qualifiers
     source          1..3682
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      37..46
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(807..825)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(808..825)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(843..859)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(843..859)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(856..878)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    867..883
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(891..897,898..915,916..921))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    936..957
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1074..1091)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1245..1833)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1325..1344)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2004..2795,2796..2864))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2627..2646
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2865..2969)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3047..3502)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3184..3205)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3396..3415
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3628..3650
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3642..3659
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3643..3659
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(3666..3682,1..3)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        join(3666..3682,1..2)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggcgaattg ggcccgacgt cgcatgctcc cggccgccat ggcggccgcg ggaattcgat
       61 taaatgacga ccgcaccata taactattcc tatattttca aatatatcat tattggtgac
      121 atgggggttg gcaagtcctg tttactccac cagttcacag aaaagaaatt tatggctgac
      181 tgtcctcata caatcggtgt tgagtttggc acacggataa ttgaagtgag tgggcagaag
      241 gtgaagcttc agatttggga cacggcaggt caggagaggt ttcgtgctgt cacacgaagc
      301 tactacagag gagccgctgg ggccctcatg gtgtatgaca tcaccaggcg aagcacgtac
      361 aatcacctta gcagctggct gactgatgcc aggaatctca ccaaccccaa tactgtcatc
      421 attctaatcg gtaacaaagc agacctagaa gcccagcgtg atgtcacata tgaagaggcc
      481 aagcagtttg ctgaagagaa cggtttgttg ttccttgagg caagtgcaaa aacaggcgag
      541 aatgtggagg atgcttttct ggaagctgcc aagaagatct accagaacat tcaggacggc
      601 agcctggacc tgaacgcagc cgagtcaggg gtccagcaca agcccaccgc cccgcaggga
      661 gggcggctca gcagcgacgc acagccccag aaagagggct gcagctgtta atgaccatgg
      721 agacttaatc actagtgaat tcgcggccgc ctgcaggtcg accatatggg agagctccca
      781 acgcgttgga tgcatagctt gagtattcta tagtgtcacc taaatagctt ggcgtaatca
      841 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
      901 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
      961 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     1021 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     1081 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     1141 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     1201 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     1261 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     1321 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     1381 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     1441 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     1501 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     1561 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     1621 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     1681 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     1741 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     1801 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     1861 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     1921 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     1981 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     2041 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     2101 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     2161 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     2221 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     2281 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     2341 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     2401 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     2461 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     2521 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     2581 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     2641 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     2701 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     2761 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     2821 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     2881 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     2941 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgatgcg
     3001 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggaaat tgtaagcgtt
     3061 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
     3121 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt
     3181 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
     3241 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg
     3301 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct
     3361 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
     3421 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt
     3481 aatgcgccgc tacagggcgc gtccattcgc cattcaggct gcgcaactgt tgggaagggc
     3541 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc
     3601 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg
     3661 aattgtaata cgactcacta ta
//
