LOCUS       sequence-168059-         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses SaBE3 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Kim YB, Komor AC, Levy JM, Packer MS, Zhao KT, Liu DR
  TITLE     Increasing the genome-targeting scope and precision of base editing 
            with engineered Cas9-cytidine deaminase fusions.
  JOURNAL   Nat Biotechnol. 2017 Feb 13. doi: 10.1038/nbt.3803.
  PUBMED    28191901
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2017 Feb 13. doi: 10.1038/nbt.3803."
COMMENT     Sequence Label: pJL-SaBE3
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     41..60
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        41..59
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             73..759
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="MSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWG
                     GRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFL
                     SRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSN
                     EAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHI
                     LWATGLK"
ORIGIN
        1 gtttagtgaa ccgtcagatc cgctagagat ccgcggccgc taatacgact cactataggg
       61 agagccgcca ccatgagctc agagactggc ccagtggctg tggaccccac attgagacgg
      121 cggatcgagc cccatgagtt tgaggtattc ttcgatccga gagagctccg caaggagacc
      181 tgcctgcttt acgaaattaa ttgggggggc cggcactcca tttggcgaca tacatcacag
      241 aacactaaca agcacgtcga agtcaacttc atcgagaagt tcacgacaga aagatatttc
      301 tgtccgaaca caaggtgcag cattacctgg tttctcagct ggagcccatg cggcgaatgt
      361 agtagggcca tcactgaatt cctgtcaagg tatccccacg tcactctgtt tatttacatc
      421 gcaaggctgt accaccacgc tgacccccgc aatcgacaag gcctgcggga tttgatctct
      481 tcaggtgtga ctatccaaat tatgactgag caggagtcag gatactgctg gagaaacttt
      541 gtgaattata gcccgagtaa tgaagcccac tggcctaggt atccccatct gtgggtacga
      601 ctgtacgttc ttgaactgta ctgcatcata ctgggcctgc ctccttgtct caacattctg
      661 agaaggaagc agccacagct gacattcttt accatcgctc ttcagtcttg tcattaccag
      721 cgactgcccc cacacattct ctgggccacc gggttgaaaa gcggcagcga gactcccggg
      781 acctcagagt ccgccacacc cgaaagtggg aaacggaact acatcctggg gcttgccatt
      841 gggataacca gcgttggcta cggaattatt gatt
//
