LOCUS       sequence-168060-         908 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses SaBE3 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Kim YB, Komor AC, Levy JM, Packer MS, Zhao KT, Liu DR
  TITLE     Increasing the genome-targeting scope and precision of base editing 
            with engineered Cas9-cytidine deaminase fusions.
  JOURNAL   Nat Biotechnol. 2017 Feb 13. doi: 10.1038/nbt.3803.
  PUBMED    28191901
REFERENCE   2  (bases 1 to 908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2017 Feb 13. doi: 10.1038/nbt.3803."
COMMENT     Sequence Label: pJL-SaBE3
FEATURES             Location/Qualifiers
     source          1..908
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1..18)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(27..47)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(60..308)
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             complement(321..386)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             complement(393..413)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 atggtgatgg tgatgatgac cggttagact ttcctcttct tcttgggaga accaccagag
       61 agcatcttaa tcttgttctc accgttgcta tcctgtatga ccagagccca aggcttgtat
      121 tcaggggcgt cgctagtcag aagcatgaca ttctcgtcgg tgctctcgtc gtaggcggtg
      181 tgcacgagta tatcgctttc cggcttgttc ccaatgactt cttccacctc ctctgggagc
      241 atgaggatgg attcctggat aaccagttgc ttaccggtct ccttttcaat aatatctgac
      301 agattagtag aaccaccaga cttgtcatcg tcatccttgt aatcgatgtc atgatcttta
      361 taatcaccgt catggtcttt gtagtcgctc gagactttcc tcttcttctt gggggatcca
      421 ccttttttaa tgatttgtgg gtgcttcttt gacttcactt catagagatt ccccaggata
      481 tcggtagagt acttttttat tgactgagtt ttagatgcga ttgtcttgat gatgcgaggc
      541 ggccgtttat cattcatgtt ttcgagatac tcccggtagg taatgtctat catattgact
      601 tcgatcctgt tcagcaagtc attgttcaca ccaatgacgc gataaagctc tccattgatc
      661 ttgatgagat cgttattata gaaggaagcg atgaactcgg cctgattgga gatcttcttc
      721 agcttcttcg cctcctcata gcactttgag tttacttcat aatagttctc ctttttgatc
      781 acgtccagat ttttcacggt gacgaattta taaaccccat tatccaggta cacgtcaaat
      841 ctataaggct tcagggagag cttcacaacc ttgtttcgag aattggggta atcgtcagtt
      901 atgtccaa
//
