LOCUS       sequence-168062-         944 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses SaKKH-BE3 in mammalian cells [Edit].
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 944)
  AUTHORS   Kim YB, Komor AC, Levy JM, Packer MS, Zhao KT, Liu DR
  TITLE     Increasing the genome-targeting scope and precision of base editing 
            with engineered Cas9-cytidine deaminase fusions.
  JOURNAL   Nat Biotechnol. 2017 Feb 13. doi: 10.1038/nbt.3803.
  PUBMED    28191901
REFERENCE   2  (bases 1 to 944)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2017 Feb 13. doi: 10.1038/nbt.3803."
COMMENT     Sequence Label: pJL-SaKKH-BE3
FEATURES             Location/Qualifiers
     source          1..944
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(25..45)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(58..306)
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             complement(319..384)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             complement(391..411)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ggtgatggtg atgatgaccg gttagacttt cctcttcttc ttgggagaac caccagagag
       61 catcttaatc ttgttctcac cgttgctatc ctgtatgacc agagcccaag gcttgtattc
      121 aggggcgtcg ctagtcagaa gcatgacatt ctcgtcggtg ctctcgtcgt aggcggtgtg
      181 cacgagtata tcgctttccg gcttgttccc aatgacttct tccacctcct ctgggagcat
      241 gaggatggat tcctggataa ccagttgctt accggtctcc ttttcaataa tatctgacag
      301 attagtagaa ccaccagact tgtcatcgtc atccttgtaa tcgatgtcat gatctttata
      361 atcaccgtca tggtctttgt agtcgctcga gactttcctc ttcttcttgg gggatccacc
      421 ttttttaatg atttgtgggt gcttctttga cttcacttca tagagattcc ccaggatatc
      481 ggtagagtac ttttttattg actgagtttt agatgcgatt gtcttgatga tgtgaggcgg
      541 ccgtttatca ttcatgtttt cgagatactc ccggtaggta atgtctatca tattgacttc
      601 gatcctgttc agcaagtcat tgttcacacc aatgacgcga taaagctctc cattgatctt
      661 gatgagatcg ttcttataga aggaagcgat gaactcggcc tgattggaga tcttcttcag
      721 cttcttcgcc tcctcatagc actttgagtt tacttcataa tagttctcct ttttgatcac
      781 gtccagattt ttcacggtga cgaatttata aaccccatta tccaggtaca cgtcaaatct
      841 ataaggcttc agggagagct tcacaacctt gtttcgagaa ttggggtaat cgtcagttat
      901 gtccaaatgc gcattaagtt tgttgccata gtactttatc tttt
//
