LOCUS       sequence-168063-         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses VQR-BE3 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Kim YB, Komor AC, Levy JM, Packer MS, Zhao KT, Liu DR
  TITLE     Increasing the genome-targeting scope and precision of base editing 
            with engineered Cas9-cytidine deaminase fusions.
  JOURNAL   Nat Biotechnol. 2017 Feb 13. doi: 10.1038/nbt.3803.
  PUBMED    28191901
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2017 Feb 13. doi: 10.1038/nbt.3803."
COMMENT     Sequence Label: pBK-VQR-BE3
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             82..768
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="MSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWG
                     GRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFL
                     SRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSN
                     EAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHI
                     LWATGLK"
ORIGIN
        1 gcagagctgg tttagtgaac cgtcagatcc gctagagatc cgcggccgct aatacgactc
       61 actataggga gagccgccac catgagctca gagactggcc cagtggctgt ggaccccaca
      121 ttgagacggc ggatcgagcc ccatgagttt gaggtattct tcgatccgag agagctccgc
      181 aaggagacct gcctgcttta cgaaattaat tgggggggcc ggcactccat ttggcgacat
      241 acatcacaga acactaacaa gcacgtcgaa gtcaacttca tcgagaagtt cacgacagaa
      301 agatatttct gtccgaacac aaggtgcagc attacctggt ttctcagctg gagcccatgc
      361 ggcgaatgta gtagggccat cactgaattc ctgtcaaggt atccccacgt cactctgttt
      421 atttacatcg caaggctgta ccaccacgct gacccccgca atcgacaagg cctgcgggat
      481 ttgatctctt caggtgtgac tatccaaatt atgactgagc aggagtcagg atactgctgg
      541 agaaactttg tgaattatag cccgagtaat gaagcccact ggcctaggta tccccatctg
      601 tgggtacgac tgtacgttct tgaactgtac tgcatcatac tgggcctgcc tccttgtctc
      661 aacattctga gaaggaagca gccacagctg acattcttta ccatcgctct tcagtcttgt
      721 cattaccagc gactgccccc acacattctc tgggccaccg ggttgaaaag cggcagcgag
      781 actcccggga cctcagagtc cgccacaccc gaaagtgata aaaagtattc tattggttta
      841 gccatcggca ctaattccgt t
//
