LOCUS       sequence-168073-         998 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses VRER-BE3 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Kim YB, Komor AC, Levy JM, Packer MS, Zhao KT, Liu DR
  TITLE     Increasing the genome-targeting scope and precision of base editing 
            with engineered Cas9-cytidine deaminase fusions.
  JOURNAL   Nat Biotechnol. 2017 Feb 13. doi: 10.1038/nbt.3803.
  PUBMED    28191901
REFERENCE   2  (bases 1 to 998)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2017 Feb 13. doi: 10.1038/nbt.3803."
COMMENT     Sequence Label: pBK-VRER-BE3
FEATURES             Location/Qualifiers
     source          1..998
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1..18)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(27..47)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(60..308)
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
ORIGIN
        1 atggtgatgg tgatgatgac cggttagact ttcctcttct tcttgggaga accaccagag
       61 agcatcttaa tcttgttctc accgttgcta tcctgtatga ccagagccca aggcttgtat
      121 tcaggggcgt cgctagtcag aagcatgaca ttctcgtcgg tgctctcgtc gtaggcggtg
      181 tgcacgagta tatcgctttc cggcttgttc ccaatgactt cttccacctc ctctgggagc
      241 atgaggatgg attcctggat aaccagttgc ttaccggtct ccttttcaat aatatctgac
      301 agattagtag aaccaccaga gtcaccccca agctgtgaca aatctatccg agtttcatat
      361 aatcccgtga tggattggtg aatcagtgtc gcgtctagca cctccttggt agatctgtac
      421 tctttgcgat ctatcgttgt gtcaaaatac ttgaatgcgg ctggagcgcc gaggttggta
      481 agagtaaaca aatggataat attttccgcc tgctcacgta tgggtttatc cctgtgcttg
      541 ttgtatgcgc ttaatacttt gtccagattg gcatcagcta ggatgactct cttactgaat
      601 tccgaaattt gctctatgat ttcgtcgaga taatgtttgt gctgctcaac aaaaagttgc
      661 ttctgttcgt tatcttcagg tgaacctttc aacttctcgt aatgggacgc taaatacagg
      721 aaattcacgt atttagacgg tagtgcgagt tcgttcccct tttgaagctc tctggcgcta
      781 gccaacatcc gttttcggcc attttctaac tcaaacagac tatactttgg tagtttaatt
      841 atgagatcct tttttacttc cttgtaacct ttcgcctcaa ggaagtcgat ggggttcttt
      901 tcaaaagacg agcgctccat aatcgttatc cccaataatt ctttgactga cttcagtttc
      961 ttggattttc ccttctcaac ttttgccact actaggac
//
