LOCUS       sequence-168077-         902 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses YE1-BE3 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Kim YB, Komor AC, Levy JM, Packer MS, Zhao KT, Liu DR
  TITLE     Increasing the genome-targeting scope and precision of base editing 
            with engineered Cas9-cytidine deaminase fusions.
  JOURNAL   Nat Biotechnol. 2017 Feb 13. doi: 10.1038/nbt.3803.
  PUBMED    28191901
REFERENCE   2  (bases 1 to 902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2017 Feb 13. doi: 10.1038/nbt.3803."
COMMENT     Sequence Label: pBK-YE1-BE3
FEATURES             Location/Qualifiers
     source          1..902
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(24..44)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(57..305)
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
ORIGIN
        1 gtgatggtga tgatgaccgg ttagactttc ctcttcttct tgggagaacc accagagagc
       61 atcttaatct tgttctcacc gttgctatcc tgtatgacca gagcccaagg cttgtattca
      121 ggggcgtcgc tagtcagaag catgacattc tcgtcggtgc tctcgtcgta ggcggtgtgc
      181 acgagtatat cgctttccgg cttgttccca atgacttctt ccacctcctc tgggagcatg
      241 aggatggatt cctggataac cagttgctta ccggtctcct tttcaataat atctgacaga
      301 ttagtagaac caccagagtc acccccaagc tgtgacaaat ctatccgagt ttcatataat
      361 cccgtgatgg attggtgaat cagtgtcgcg tctagcacct ccttggtaga agtgtatcgt
      421 ttgcgatcta tcgttgtgtc aaaatacttg aatgcggctg gagcgccgag gttggtaaga
      481 gtaaacaaat ggataatatt ttccgcctgc tcacgtatgg gtttatccct gtgcttgttg
      541 tatgcgctta atactttgtc cagattggca tcagctagga tgactctctt actgaattcc
      601 gaaatttgct ctatgatttc gtcgagataa tgtttgtgct gctcaacaaa aagttgcttc
      661 tgttcgttat cttcaggtga acctttcaac ttctcgtaat gggacgctaa atacaggaaa
      721 ttcacgtatt tagacggtag tgcgagttcg ttcccctttt gaagctctcc ggcgctagcc
      781 aacatccgtt ttcggccatt ttctaactca aacagactat actttggtag tttaattatg
      841 agatcctttt ttacttcctt gtaacctttc gcctcaagga agtcgatggg gttcttttca
      901 aa
//
