LOCUS       sequence-168078-         935 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive CRISPR interference (CRISPRi) knock in construct into 
            the AAVS1 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Mandegar MA, Huebsch N, Frolov EB, Shin E, Truong A, Olvera MP, Chan
            AH, Miyaoka Y, Holmes K, Spencer CI, Judge LM, Gordon DE, Eskildsen 
            TV, Villalta JE, Horlbeck MA, Gilbert LA, Krogan NJ, Sheikh SP, 
            Weissman JS, Qi LS, So PL, Conklin BR
  TITLE     CRISPR Interference Efficiently Induces Specific and Reversible Gene
            Silencing in Human iPSCs.
  JOURNAL   Cell Stem Cell. 2016 Apr 7;18(4):541-53. doi: 
            10.1016/j.stem.2016.01.022. Epub 2016 Mar 10.
  PUBMED    26971820
REFERENCE   2  (bases 1 to 935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2016.01.022"; journalName: "Cell Stem Cell"; date: 
            "2016-04-7- 7"; volume: "18"; issue: "4"; pages: "541-53"
COMMENT     Sequence Label: pAAVS1-NC-CRISPRi (Gen3)
FEATURES             Location/Qualifiers
     source          1..935
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             32..217
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEP"
ORIGIN
        1 catggatgct aagtcactaa ctgcctggtc ccggacactg gtgaccttca aggatgtatt
       61 tgtggacttc accagggagg agtggaagct gctggacact gctcagcaga tcgtgtacag
      121 aaatgtgatg ctggagaact ataagaacct ggtttccttg ggttatcagc ttactaagcc
      181 agatgtgatc ctccggttgg agaagggaga agagcccggc ggttccggcg gagggtcgat
      241 ggacaagaag tacagcatcg gcctggccat cggcaccaac tctgtgggct gggccgtgat
      301 caccgacgag tacaaggtgc ccagcaagaa attcaaggtg ctgggcaaca ccgaccggca
      361 cagcatcaag aagaacctga tcggcgccct gctgttcgac agcggagaaa cagccgaggc
      421 cacccggctg aagagaaccg ccagaagaag atacaccaga cggaagaacc ggatctgcta
      481 tctgcaagag atcttcagca acgagatggc caaggtggac gacagcttct tccacagact
      541 ggaagagtcc ttcctggtgg aagaggataa gaagcacgag cggcacccca tcttcggcaa
      601 catcgtggac gaggtggcct accacgagaa gtaccccacc atctaccacc tgagaaagaa
      661 actggtggac agcaccgaca aggccgacct gcggctgatc tatctggccc tggcccacat
      721 gatcaagttc cggggccact tcctgatcga gggcgacctg aaccccgaca acagcgacgt
      781 ggacaagctg ttcatccagc tggtgcagac ctacaaccag ctgttcgagg aaaaccccat
      841 caacgccagc ggcgtggacg ccaaggccat cctgtctgcc agactgagca agagcagacg
      901 gctggaaaat ctgatcgccc agctgcccgg cgaga
//
