LOCUS       sequence-168080-         892 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses EE-BE3 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Kim YB, Komor AC, Levy JM, Packer MS, Zhao KT, Liu DR
  TITLE     Increasing the genome-targeting scope and precision of base editing 
            with engineered Cas9-cytidine deaminase fusions.
  JOURNAL   Nat Biotechnol. 2017 Feb 13. doi: 10.1038/nbt.3803.
  PUBMED    28191901
REFERENCE   2  (bases 1 to 892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2017 Feb 13. doi: 10.1038/nbt.3803."
COMMENT     Sequence Label: pBK-EE-BE3
FEATURES             Location/Qualifiers
     source          1..892
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     45..64
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        45..63
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gctggtttag tgaaccgtca gatccgctag agatccgcgg ccgctaatac gactcactat
       61 agggagagcc gccaccatga gctcagagac tggcccagtg gctgtggacc ccacattgag
      121 acggcggatc gagccccatg agtttgaggt attcttcgat ccgagagagc tccgcaagga
      181 gacctgcctg ctttacgaaa ttaattgggg gggccggcac tccatttggc gacatacatc
      241 acagaacact aacaagcacg tcgaagtcaa cttcatcgag aagttcacga cagaaagata
      301 tttctgtccg aacacaaggt gcagcattac ctggtttctc agctggagcc catgcggcga
      361 atgtagtagg gccatcactg aattcctgtc aaggtatccc cacgtcactc tgtttattta
      421 catcgcaagg ctgtaccacc acgctgaccc cgagaatcga caaggcctgg aagatttgat
      481 ctcttcaggt gtgactatcc aaattatgac tgagcaggag tcaggatact gctggagaaa
      541 ctttgtgaat tatagcccga gtaatgaagc ccactggcct aggtatcccc atctgtgggt
      601 acgactgtac gttcttgaac tgtactgcat catactgggc ctgcctcctt gtctcaacat
      661 tctgagaagg aagcagccac agctgacatt ctttaccatc gctcttcagt cttgtcatta
      721 ccagcgactg cccccacaca ttctctgggc caccgggttg aaaagcggca gcgagactcc
      781 cgggacctca gagtccgcca cacccgaaag tgataaaaag tattctattg gtttagccat
      841 cggcactaat tccgttggat gggctgtcat aaccgatgaa tacaaagtac ct
//
