LOCUS       sequence-168081-         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive CRISPR interference (CRISPRi) knock in construct into 
            the AAVS1 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Mandegar MA, Huebsch N, Frolov EB, Shin E, Truong A, Olvera MP, Chan
            AH, Miyaoka Y, Holmes K, Spencer CI, Judge LM, Gordon DE, Eskildsen 
            TV, Villalta JE, Horlbeck MA, Gilbert LA, Krogan NJ, Sheikh SP, 
            Weissman JS, Qi LS, So PL, Conklin BR
  TITLE     CRISPR Interference Efficiently Induces Specific and Reversible Gene
            Silencing in Human iPSCs.
  JOURNAL   Cell Stem Cell. 2016 Apr 7;18(4):541-53. doi: 
            10.1016/j.stem.2016.01.022. Epub 2016 Mar 10.
  PUBMED    26971820
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2016.01.022"; journalName: "Cell Stem Cell"; date: 
            "2016-04-7- 7"; volume: "18"; issue: "4"; pages: "541-53"
COMMENT     Sequence Label: pAAVS1-NC-CRISPRi (Gen3)
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gccgcctctc tcggccttgg tcagattgtc gaacttcctc tgggtaatca gcttggcatt
       61 cagcagctgg cgccagtagt tcttcatctt cttcacgacc tcttcggagg gcacgttgtc
      121 gctcttgccc cggttcttgt cgctccgagt cagcactttg ttatcgatgg agtcgtcctt
      181 cagaaagctc tgaggcacga tagcgtccac atcgtagtcg gacagccggt tgatgtccag
      241 ttcctggtcc acgtacatat cccgcccatt ctgcaggtag tacaggtaca gcttctcgtt
      301 ctgcagctgg gtgttttcca cggggtgttc tttcaggatc tggctgccca gctctttgat
      361 gccctcttcg atccgcttca ttctctcgcg gctgttcttc tgtcccttct gggtggtctg
      421 gttctctctg gccatttcga tcacgatgtt ctcgggcttg tgccggccca tcactttcac
      481 gagctcgtcc accaccttca ctgtctgcag gatgcccttc ttaatggcgg ggctgccggc
      541 cagattggca atgtgctcgt gcaggctatc gccctggccg gacacctggg ctttctggat
      601 gtcctcttta aaggtcaggc tgtcgtcgtg gatcagctgc atgaagtttc tgttggcgaa
      661 gccgtcggac ttcaggaaat ccaggattgt cttgccggac tgcttgtccc ggatgccgtt
      721 gatcagcttc cggctcagcc tgccccagcc ggtgtatctc cgccgcttca gctgcttcat
      781 cactttgtcg tcgaacaggt gggcataggt tttcagccgt tcctcgatca tctctctgtc
      841 ctcaaacagt gtca
//
