LOCUS       sequence-168082-        4038 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing IDH1, part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4038)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4038)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_IDH1_p.R132H
FEATURES             Location/Qualifiers
     source          1..4038
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      32..620
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     521..540
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     774..791
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(950..977)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1069..1155)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1204..1226
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1218..1235
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1219..1235
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1251..1350
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2599..2698)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2715..2734)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2716..2734)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2739..2755)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2739..2755)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3186..3977
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
       61 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
      121 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
      181 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
      241 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
      301 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
      361 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
      421 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
      481 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
      541 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
      601 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
      661 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
      721 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
      781 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
      841 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
      901 acgcaattaa tacgcgtacc gctagccagg aagagtttgt agaaacgcaa aaaggccatc
      961 cgtcaggatg gccttctgct tagtttgatg cctggcagtt tatggcgggc gtcctgcccg
     1021 ccaccctccg ggccgttgct tcacaacgtt caaatccgct cccggcggat ttgtcctact
     1081 caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt ccgactgagc
     1141 ctttcgtttt atttgatgcc tggcagttcc ctactctcgc gttaacgcta gcatggatgt
     1201 tttcccagtc acgacgttgt aaaacgacgg ccagtcttaa gctcgggccc caaataatga
     1261 ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa tgctttttta
     1321 taatgccaac tttgtacaaa aaagttggca ccatgtccaa aaaaatcagt ggcggttctg
     1381 tggtagagat gcaaggagat gaaatgacac gaatcatttg ggaattgatt aaagagaaac
     1441 tcatttttcc ctacgtggaa ttggatctac atagctatga tttaggcata gagaatcgtg
     1501 atgccaccaa cgaccaagtc accaaggatg ctgcagaagc tataaagaag cataatgttg
     1561 gcgtcaaatg tgccactatc actcctgatg agaagagggt tgaggagttc aagttgaaac
     1621 aaatgtggaa atcaccaaat ggcaccatac gaaatattct gggtggcacg gtcttcagag
     1681 aagccattat ctgcaaaaat atcccccggc ttgtgagtgg atgggtaaaa cctatcatca
     1741 taggtcatca tgcttatggg gatcaataca gagcaactga ttttgttgtt cctgggcctg
     1801 gaaaagtaga gataacctac acaccaagtg acggaaccca aaaggtgaca tacctggtac
     1861 ataactttga agaaggtggt ggtgttgcca tggggatgta taatcaagat aagtcaattg
     1921 aagattttgc acacagttcc ttccaaatgg ctctgtctaa gggttggcct ttgtatctga
     1981 gcaccaaaaa cactattctg aagaaatatg atgggcgttt taaagacatc tttcaggaga
     2041 tatatgacaa gcagtacaag tcccagtttg aagctcaaaa gatctggtat gagcataggc
     2101 tcatcgacga catggtggcc caagctatga aatcagaggg aggcttcatc tgggcctgta
     2161 aaaactatga tggtgacgtg cagtcggact ctgtggccca agggtatggc tctctcggca
     2221 tgatgaccag cgtgctggtt tgtccagatg gcaagacagt agaagcagag gctgcccacg
     2281 ggactgtaac ccgtcactac cgcatgtacc agaaaggaca ggagacgtcc accaatccca
     2341 ttgcttccat ttttgcctgg accagagggt tagcccacag agcaaagctt gataacaata
     2401 aagagcttgc cttctttgca aatgctttgg aagaagtctc tattgagaca attgaggctg
     2461 gcttcatgac caaggacttg gctgcttgca ttaaaggttt acccaatgtg caacgttctg
     2521 actacttgaa tacatttgag ttcatggata aacttggaga aaacttgaag atcaaactag
     2581 ctcaggccaa actttaattg ccaactttct tgtacaaagt tggcattata agaaagcatt
     2641 gcttatcaat ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgcc
     2701 atccagctga tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc
     2761 tctggcccgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat
     2821 gcctcctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc aaggttgccg
     2881 ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa gcctgttcgg
     2941 ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg
     3001 aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg
     3061 gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt
     3121 tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa
     3181 acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg
     3241 tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg
     3301 atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg
     3361 atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag
     3421 agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt
     3481 ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg
     3541 caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa
     3601 gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg
     3661 aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact
     3721 gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa
     3781 ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc
     3841 agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct
     3901 tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca
     3961 aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac gtgagttacg
     4021 cgtcgttcca ctgagcgt
//
